Molecular characterization of Xiphinema brevicollum (Nematoda: Longidoridae) from the Czech Republic

被引：8
作者：
Kumari, Shesh [1 ]
Decraemer, Wilfrida [2 ,3 ]
De Luca, Francesca [4 ]
机构：
[1] Crop Res Inst, Div Plant Hlth, Prague 16106 6, Czech Republic
[2] Royal Belgian Inst Nat Sci, Dept Invertebrates, B-1000 Brussels, Belgium
[3] Univ Ghent, Dept Biol, B-9000 Ghent, Belgium
[4] CNR, Ist Protez Piante, I-70126 Bari, Italy
来源：
EUROPEAN JOURNAL OF PLANT PATHOLOGY | 2010年 / 128卷 / 02期
关键词：
Xiphinema inaequale; Xiphinema italiae; Xiphinema lambertii; Nematode; Mitochondrial DNA; Ribosomal DNA; POPULATION GENETIC-STRUCTURE; AMERICANUM-GROUP;
D O I：
10.1007/s10658-010-9651-8
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
Populations of Xiphinema brevicollum occurring in the Czech Republic were described morphologically and molecularly. Published species-specific primer set BL18 and BV3 was used to amplify three populations of X. brevicollum from the Czech Republic. These primers were tested against 9 species of Xiphinema and 11 species of Longidorus. Amplification was also observed for X. inaequale, X. italiae and X. lambertii. Three additional markers, mitochondrial cytochrome c oxidase subunit 1, ribosomal D2/D3 expansion segment of the 28S gene and 18S gene, were amplified and sequenced for X. brevicollum, X. inaequale and X. lambertii belonging to X. americanum-group. Comparison of cox1 sequences of X. brevicollum from the Czech Republic with X. taylori from the Slovak Republic (accession number AM086702) suggested that these populations represent the same species.
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页码：243 / 250
页数：8
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