The detection of bacteria on Microarrays using up-converting phosphor nanoparticles as fluorescent labels

被引：1
作者：
Guo, Yonggang [1 ,2 ]
Deng, Wei [3 ,4 ]
Guo, Min [3 ]
Chen, Depu [4 ]
Cheng, Jing [1 ,2 ,3 ]
机构：
[1] Tsinghua Univ, Med Syst Biol Res Ctr, Beijing 100084, Peoples R China
[2] Tsinghua Univ, Dept Biol Sci & Biotechnol, Beijing 100084, Peoples R China
[3] Beijing Biochip Technol, Natl Engn Res Ctr, Beijing, Peoples R China
[4] Tsinghua Univ, Dept Chem, Beijing, Peoples R China
来源：
2006 1ST IEEE INTERNATIONAL CONFERENCE ON NANO/MICRO ENGINEERED AND MOLECULAR SYSTEMS, VOLS 1-3 | 2006年
关键词：
nanoparticals; UCP; streptavidin; microarray;
D O I：
10.1109/NEMS.2006.334838
中图分类号：
TM [电工技术]; TN [电子技术、通信技术];
学科分类号：
0808 ; 0809 ;
摘要：
Nanoparticles containing the infrared-to-visible up-converting phosphors (UCP) ytterbium and erbium and co-doped with sodium yttriurn fluoride were synthesized with diameters ranging from 30nm to 50nm. These high luminescence efficient UCP beads were employed as detection labels and applied in microarrays for the detection of specific nucleic acid hybrids to identify bacteria for the first time. The low cost of UCP nanoparticles and their detection equipment may facilitate their broad application in areas requiring large numbers of assays such as clinical diagnostic and environmental monitoring.
引用
收藏
页码：550 / +
页数：3
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