Sensitive determination of ketoprofen using flow injection with chemiluminescence detection

A rapid chemiluminescence method is described for the determination of ketoprofen by combining the flow injection technique and its sensitizing effect on the weak chemiluminescence reaction between sulfite and acidic permanganate. The optimum conditions for the chemiluminescence emission were intensity. A mechanism for the chemiluminescence reaction has been proposed on the basis of chemiluminescent spectra. Ketoprofen can be determined over the concentration range of 5.0 x 10(-8) to 3.0 x 10(-6) mol/L with a correlation coefficient of 0.9999 and a detection limit of 2.0 x 10(-8) mol/L (3 sigma). The relative standard deviation (R.S.D.) for 15 repetitive determinations of 1.0 x 10(-6) mol/L ketoprofen is 0.8%. The utility of this method was demonstrated by determining ketoprofen in capsules and human urine sample. (c) 2007 Elsevier B.V. All rights reserved.