Kurarinone isolated from Sophora flavescens Ait inhibited MCP-1-induced chemotaxis

被引：24

作者：

Lee, SW ^{[1
]}

Lee, HS ^{[1
]}

Nam, JY ^{[1
]}

Kwon, OE ^{[1
]}

Baek, JA ^{[1
]}

Chang, JS ^{[1
]}

Rho, MC ^{[1
]}

Kim, YK ^{[1
]}

机构：

[1] Korea Res Inst Biosci & Biotechnol, Lab Lipid Metab, Taejon 305333, South Korea

来源：

JOURNAL OF ETHNOPHARMACOLOGY | 2005年 / 97卷 / 03期

关键词：

Sophora flavescens Ait; kurarinone; active-guided fractionations; monocyte chemoattractant protein-1; CC chemokine receptor;

D O I：

10.1016/j.jep.2004.12.006

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The accumulation of circulating monocytes in the arterial wall is an early in atherosclerotic plaque formation. Monocyte chemoattractant protein-1 (MCP-1) promotes the migration of monocytes and would play a role in the development of atherosclerotic lesions. Searching for inhibitors of MCP-1-induced cell migration from natural sources, we isolated one active compound through active-guided fractionations from the MeOH extracts of Sophora flavescens Air (Leguminosae). On the basis of spectral evidence, the structure of active compound was identified as kurarinone. It inhibited the migration of THP-1 cells induced by MCP-1 with IC50 value of 19.2 mu g/mL. In addition, it inhibited the binding of MCP-1 to THP-1 cells and phosphorylation of p42/44 MARK. (c) 2005 Elsevier Ireland Ltd. All rights reserved.

引用

页码：515 / 519

页数：5

共 20 条

[1] Decreased lesion formation in CCR2-/- mice reveals a role for chemokines in the initiation of atherosclerosis [J].

Boring, L ;

Gosling, J ;

Cleary, M ;

Charo, IF .

NATURE, 1998, 394 (6696) :894-897

[2]

BROWN Z, 1994, AM J PATHOL, V145, P913

[3] Signal transduction involved in MCP-1-mediated monocytic transendothelial migration [J].

Cambien, B ;

Pomeranz, M ;

Millet, MA ;

Rossi, B ;

Schmid-Alliana, A .

BLOOD, 2001, 97 (02) :359-366

[4] MOLECULAR-CLONING AND FUNCTIONAL EXPRESSION OF 2 MONOCYTE CHEMOATTRACTANT PROTEIN-1 RECEPTORS REVEALS ALTERNATIVE SPLICING OF THE CARBOXYL-TERMINAL TAILS [J].

CHARO, IF ;

MYERS, SJ ;

HERMAN, A ;

FRANCI, C ;

CONNOLLY, AJ ;

COUGHLIN, SR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (07) :2752-2756

[5] A SENSITIVE ELISA FOR THE DETECTION OF HUMAN MONOCYTE CHEMOATTRACTANT PROTEIN-1 (MCP-1) [J].

EVANOFF, HL ;

BURDICK, MD ;

MOORE, SA ;

KUNKEL, SL ;

STRIETER, RM .

IMMUNOLOGICAL INVESTIGATIONS, 1992, 21 (01) :39-45

[6]

Ha TJ, 2001, B KOR CHEM SOC, V22, P97

[7]

JAMES JO, 2002, TRENDS PHARMACOL SCI, V23, P459

[8] Cytotoxic lavandulyl flavanones from Sophora flavescens [J].

Kang, TH ;

Jeong, SJ ;

Ko, WG ;

Kim, NY ;

Lee, BH ;

Inagaki, M ;

Miyamoto, T ;

Higuchi, R ;

Kim, YC .

JOURNAL OF NATURAL PRODUCTS, 2000, 63 (05) :680-681

[9] Nonrequirement of continuous stimulation with MCP-1 for cell migration and determination of directional migration by initial stimulation with chemokine [J].

Kito, K ;

Nishida, K .

EXPERIMENTAL CELL RESEARCH, 2002, 281 (01) :157-166

[10] Inhibition of phospholipase C gamma(1) by the prenylated flavonoids from Sophoro flavescens [J].

Lee, HS ;

Ko, HR ;

Ryu, SY ;

Oh, WK ;

Kim, BY ;

Ahn, SC ;

Mheen, TI ;

Ahn, JS .

PLANTA MEDICA, 1997, 63 (03) :266-268

← 1 2 →