Effects of Cadmium Sulfate on the Brown Garden Snail Cornu aspersum: Implications for DNA Methylation

被引:7
|
作者
Georgescu, Marius [1 ,2 ]
Draghici, George Andrei [3 ,4 ]
Oancea, Eliza-Florentina [5 ]
Dehelean, Cristina Adriana [3 ,4 ]
Soica, Codruta [3 ,4 ]
Vladut, Nicolae-Valentin [6 ]
Nica, Dragos Vasile [3 ,6 ]
机构
[1] Victor Babes Univ Med & Pharm Timisoara, Dept Funct Sci, Physiol Discipline, Eftimie Murgu Sq 2, Timisoara 300041, Romania
[2] Victor Babes Univ Med & Pharm Timisoara, Ctr Immunophysiol & Biotechnol CIFBIOTEH, Eftimie Murgu Sq 2, Timisoara 300041, Romania
[3] Victor Babes Univ Med & Pharm Timisoara, Fac Pharm, Eftimie Murgu Sq 2, Timisoara 300041, Romania
[4] Victor Babes Univ Med & Pharm Timisoara, Fac Pharm, Res Ctr Pharmaco Toxicol Evaluat, Eftimie Murgu Sq 2, Timisoara 300041, Romania
[5] Vasile Goldi Western Univ Arad, Fac Pharm, B Dul Revolutiei 94, Arad 310025, Romania
[6] INMA Bucharest, Bulevardul Ion Lonescu Brad 6, Bucureti 077190, Romania
关键词
cadmium exposure; DNA methylation; 5-methylcytosine; metallothioneins; cadmium sulfate; CdMT gene; land snails; hepatopancreas; HELIX-ASPERSA; EXPOSURE; CD; EPIGENETICS; PROMOTER; POMATIA; COPPER;
D O I
10.3390/toxics9110306
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
An extensive literature exists regarding the cellular, physiological, and genetic effects of cadmium (Cd)-A highly toxic, but commonly used trace metal in modern industry. However, limited data are available on its epigenetic effects, especially for terrestrial sentinel invertebrates. We determined Cd retention, total DNA methylation, and the methylation status of 5 & PRIME; end of the Cd-MT gene in the hepatopancreas of the brown garden snail, Cornu aspersum, fed Cd sulfate for four weeks. Bodyweight changes and survival were also measured. Hepatopancreas cadmium increased in a dose-dependent manner from the third-lowest dose onward, with very large amounts being found for the highest treatment group. However, no mortalities occurred, irrespective of dietary Cd dose. We identified significant genome-wide hypermethylation in specimens given the highest dose, which overlapped with a significant bodyweight decrease. The Cd-MT gene showed an unmethylated 5 & PRIME; end of the Cd-MT gene and this status was not affected by cadmium exposure. Hepatopancreas DNA methylation is as sensitive as bodyweight to non-lethal concentrations of dietary Cd given as cadmium sulfate but less responsive than tissue accumulation. Such an exposure event, by contrast, does not affect the methylation status of the Cd-MT gene 5 & PRIME; end.
引用
收藏
页数:11
相关论文
共 47 条
  • [1] The Developmental Genetic Basis of Appendage Patterning in the Brown Garden Snail, Cornu aspersum
    Abshire, Kaitlyn
    Laumer, Ethan
    Neu, Sophie
    Wood, Charlotte
    Sharma, Prashant
    INTEGRATIVE AND COMPARATIVE BIOLOGY, 2024, 64 : S2 - S2
  • [2] Stage- and weather-dependent dispersal in the brown garden snail Cornu aspersum
    Dahirel, Maxime
    Ansart, Armelle
    Madec, Luc
    POPULATION ECOLOGY, 2014, 56 (01) : 227 - 237
  • [3] Responsiveness of metallothionein and hemocyanin genes to cadmium and copper exposure in the garden snail Cornu aspersum
    Pedrini-Martha, Veronika
    Schnegg, Raimund
    Schaefer, Gabriela Giannina
    Lieb, Bernhard
    Salvenmoser, Willi
    Dallinger, Reinhard
    JOURNAL OF EXPERIMENTAL ZOOLOGY PART A-ECOLOGICAL AND INTEGRATIVE PHYSIOLOGY, 2021, 335 (02) : 228 - 238
  • [4] Development of Crenosoma vulpis in the common garden snail Cornu aspersum: implications for epidemiological studies
    Colella, Vito
    Mutafchiev, Yasen
    Cavalera, Maria Alfonsa
    Giannelli, Alessio
    Lia, Riccardo Paolo
    Dantas-Torres, Filipe
    Otranto, Domenico
    PARASITES & VECTORS, 2016, 9
  • [5] Identification and characterisation of anti - Pseudomonas aeruginosa proteins in mucus of the brown garden snail, Cornu aspersum
    Pitt, S. J.
    Hawthorne, J. A.
    Garcia-Maya, M.
    Alexandrovich, A.
    Symonds, R. C.
    Gunn, A.
    BRITISH JOURNAL OF BIOMEDICAL SCIENCE, 2019, 76 (03) : 129 - 136
  • [6] Development of Crenosoma vulpis in the common garden snail Cornu aspersum: implications for epidemiological studies
    Vito Colella
    Yasen Mutafchiev
    Maria Alfonsa Cavalera
    Alessio Giannelli
    Riccardo Paolo Lia
    Filipe Dantas-Torres
    Domenico Otranto
    Parasites & Vectors, 9
  • [7] Isolation of cellulase enzyme from brown garden snail (Cornu aspersum) for the saccharification of waste paper materials
    Ndlovu, T. M.
    Van Wyk, J. P. H.
    METHODSX, 2019, 6 : 1030 - 1035
  • [8] Coordinating associative and ecological accounts of learning in the garden snail Cornu aspersum
    Loy, I.
    Alvarez, B.
    Strempler-Rubio, E. C.
    Rodriguez, M.
    BEHAVIOURAL PROCESSES, 2017, 139 : 26 - 32
  • [9] Structural and conformational stability of hemocyanin from the garden snail Cornu aspersum
    Dolashki, Aleksandar
    Velkova, Lyudmila
    Voelter, Wolfgang
    Dolashka, Pavlina
    ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES, 2019, 74 (5-6): : 113 - 123
  • [10] Effect of boiling on chemical composition of small brown snail (Cornu aspersum aspersum) meat
    Ligaszewski, Maciej
    Surowka, Krzysztof
    Szymczyk, Beata
    Pol, Przemyslaw
    Anthony, Barbara
    ANNALS OF ANIMAL SCIENCE, 2024, 24 (01) : 257 - 268