Separation of polysorbates by liquid chromatography on a HILIC column and identification of peaks by MALDI-TOF MS

被引：21

作者：

Abrar, Shazia ^{[1
]}

Trathnigg, Bernd ^{[1
]}

机构：

[1] Cent Polymer Labs CePoL, NAWI Graz, Cent Polymer Lab Mol Characterist CePol MC, A-8010 Graz, Austria

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2011年 / 400卷 / 07期

关键词：

Polysorbate; Tween; Liquid chromatography under critical conditions; HILIC; MALDI-TOF MS; HYDROPHILIC INTERACTION CHROMATOGRAPHY; EXCLUSION-ADSORPTION CHROMATOGRAPHY; 2-DIMENSIONAL SEPARATION; NONIONIC SURFACTANTS; ISOCRATIC SEPARATION; ALCOHOL ETHOXYLATES; BLOCK-COPOLYMERS; PHASE; MACROMOLECULES; SORBITOL;

D O I：

10.1007/s00216-011-4933-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Polysorbates can be separated according to their functionality and architecture by liquid chromatography on a hydrophilic interaction chromatography (HILIC) column in acetone-water mobile phases containing 90-97% acetone. The different polymer homologous series are separated according to the number of terminal hydroxy groups and elute as narrow peaks. The hydrophilic part (ethoxylates of sorbitan, isosorbide, and poly(ethyleneglycol)) and amphiphilic funtionalites (ethoxylated mono-, di-, tri-, and tetraesters) were separated by HILIC mode of high-performance liquid chromatography (HPLC). All these separated functionaities are identified and confirmed by matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy (MALDI-TOF MS). This combination of HPLC and MALDI-TOF MS has been proven to be an excellent tool for the characterization of heterogenous complex samples.

引用

页码：2119 / 2130

页数：12

共 27 条

[1] Characterization of Commercial Polysorbates Using Different Chromatographic Techniques [J].

Abrar, S. ;

Trathnigg, B. .

TENSIDE SURFACTANTS DETERGENTS, 2009, 46 (05) :280-288

[2]

ABRAR S, 2011, J CHROMAT A IN PRESS

[3]

ABRAR S, 2011, ANAL BIOANAL C UNPUB

[4]

Ayorinde FO, 1999, RAPID COMMUN MASS SP, V13, P1124, DOI 10.1002/(SICI)1097-0231(19990630)13:12<1124::AID-RCM624>3.0.CO

[5]

2-O

[6] Surfactants from biomass: A two-step cascade reaction for the synthesis of sorbitol fatty acid esters using solid acid catalysts [J].

Corma, Avelino ;

Hamid, Sharifah B. A. ;

Iborra, Sara ;

Velty, Alexandra .

CHEMSUSCHEM, 2008, 1 (1-2) :85-90

[7]

Cottrell T., 2007, EMULSIFIERS FOOD TEC, P162

[8] Analysis of aliphatic alcohol ethoxylates in terms of alkyl and ethylene oxide chain lengths by reversed-phase liquid chromatography with evaporative light scattering detection [J].

Cretier, G ;

Podevin, C ;

Rocca, JL .

JOURNAL OF CHROMATOGRAPHY A, 2000, 874 (02) :305-310

[9] Composition analysis of two batches of polysorbate 60 using MS and NMR techniques [J].

Dang, HV ;

Gray, AI ;

Watson, D ;

Bates, CD ;

Scholes, P ;

Eccleston, GM .

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2006, 40 (05) :1155-1165

[10] Investigating the molecular heterogeneity of polysorbate emulsifiers by MALDI-TOF MS [J].

Frison-Norrie, S ;

Sporns, P .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2001, 49 (07) :3335-3340

← 1 2 3 →