Effect of daily oscillation in temperature and increased suspended sediment on growth and smolting in juvenile chinook salmon, Oncorhynchus tshawytscha

We examined the effect of temperature oscillation and increased suspended sediment concentration on growth and smolting in juvenile ocean-type chinook salmon (Oncorhynchus tshawytscha). Fish were ponded on February 26; each treatment group had three replicates of 250 fish. Mean temperatures for the entire experiment were 12.3 degrees C for all tanks with a total of 1348 and 1341 degree days for the constant temperature and oscillating temperature tanks, respectively. Daily fluctuation in temperature averaged 7.5 degrees C in the variable temperature groups and less than 1 degrees C for the constant temperature group. Starting on April 5, bentonite clay was added each day to tanks as a pulse event to achieve a suspended sediment concentration of 200 mg l(-1); clay cleared from the tanks within approximately 8 h. Fish were sampled at approximately two week intervals from ponding until mid-June. On the last sample date, June 12, a single gill arch was removed and fixed for histological examination of gill morphology. By early May, significant differences were seen in size between the groups; control>temperature=sediment>(temperature x sediment). This relationship was consistent throughout the experiment except for the last sample date when the temperature group had a mean weight significantly greater than the sediment group. Gill Na+,K+-ATPase activity was not affected by daily temperature oscillations, but groups subjected to increased suspended sediment had significantly lower enzyme activities compared to controls. Mean cell size for gill chloride cells did not differ between groups. Plasma cortisol increased significantly during the spring, but there were no significant differences between groups. (C) 2007 Elsevier B.V. All rights reserved.