Evaluation of filter paper as a means to transport inactivated bacteria for identification using the MALDI-TOF MS system

被引:6
作者
Carneiro, M. S. [1 ,2 ]
Fracasso, A. [1 ]
Lovison, O. V. A. [1 ,2 ]
Barreto, F. [3 ]
Barth, A. L. [1 ,2 ]
机构
[1] HCPA, Lab Pesquisa Resistencia Bacteriana LABRESIS, CPE, R Ramiro Barcelos 2350, BR-90035903 Porto Alegre, RS, Brazil
[2] Univ Fed Rio Grande do Sul, Progmma Posgrad Ciencias Farmaceut PPGCF, Fac Farmcicia, Av Ipiranga 2752, Porto Alegre, RS, Brazil
[3] LFDA, Estr Ponta Grossa 3036, BR-91780580 Porto Alegre, RS, Brazil
关键词
Filter paper; MALDI-TOF MS; Transport bacteria; SAMPLES; STORAGE;
D O I
10.1016/j.mimet.2020.105863
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A novel approach to transport inactivated bacteria in filter paper for identification in the MALDI-TOF MS was evaluated. Seventy four bacterial isolates were evaluated and the approach presented sensitivity of 97.3% and specificity of 100%. Inactivated bacteria in filter paper are a safer alternative to transport bacteria for MALDI-TOF MS identification.
引用
收藏
页数:4
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共 7 条
[1]   Use of serum and blood samples on filter paper to improve the surveillance of dengue in Pacific Island Countries [J].
Aubry, Maite ;
Roche, Claudine ;
Dupont-Rouzeyrol, Myrielle ;
Aaskov, John ;
Viallon, Jerome ;
Marfel, Maria ;
Lalita, Paul ;
Elbourne-Duituturaga, Salanieta ;
Chanteau, Suzanne ;
Musso, Didier ;
Pavlin, Boris I. ;
Harrison, Dustin ;
Kool, Jacob L. ;
Cao-Lormeau, Van-Mai .
JOURNAL OF CLINICAL VIROLOGY, 2012, 55 (01) :23-29
[2]   Storage and stability of IgG and IgM monoclonal antibodies dried on filter paper and utility in Neisseria meningitidis serotyping by Dot-blot ELISA [J].
Ferraz, Aline S. ;
Belo, Elza F. T. ;
Coutinho, Ligia M. C. C. ;
Oliveira, Ana P. ;
Carmo, Andreia M. S. ;
Franco, Daniele L. ;
Ferreira, Tatiane ;
Yto, Andre Y. ;
Machado, Marta S. F. ;
Scola, Monica C. G. ;
De Gaspari, Elizabeth .
BMC INFECTIOUS DISEASES, 2008, 8 (1)
[3]   Recent Advances and Ongoing Challenges in the Diagnosis of Microbial Infections by MALDI-TOF Mass Spectrometry [J].
Florio, Walter ;
Tavanti, Arianna ;
Barnini, Simona ;
Ghelardi, Emilia ;
Lupetti, Antonella .
FRONTIERS IN MICROBIOLOGY, 2018, 9
[4]   Use of cerebrospinal fluid and serum samples impregnated on FTA™ Elute filter paper for the diagnosis of infections caused by Neisseria meningitidis, Streptococcus pneumoniae and Haemophilus influenzae [J].
Fukasawa, Lucila Okuyama ;
Goncalves, Maria Gisele ;
Higa, Fabio Takenori ;
Castilho, Euclides Ayres ;
Ibarz-Pavon, Ana Belen ;
Sacchi, Claudio Tavares .
PLOS ONE, 2017, 12 (02)
[5]   Routine identification of microorganisms by matrix-assisted laser desorption ionization time-of-flight mass spectrometry: Success rate, economic analysis, and clinical outcome [J].
Ge, Mao-Cheng ;
Kuo, An-Jing ;
Liu, Kuei-Lan ;
Wen, Ying-Hao ;
Chia, Ju-Hsin ;
Chang, Pi-Yueh ;
Lee, Ming-Hsun ;
Wu, Tsu-Lan ;
Chang, Shih-Cheng ;
Lu, Jang-Jih .
JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION, 2017, 50 (05) :662-668
[6]   Inactivation, storage, and PCR detection of mycoplasma on FTA® filter paper [J].
Moscoso, H ;
Thayer, SG ;
Hofacre, CL ;
Meven, SH .
AVIAN DISEASES, 2004, 48 (04) :841-850
[7]   Storage of bovine viral diarrhoea virus samples on filter paper and detection of viral RNA by a RT-PCR method [J].
Vilcek, S ;
Strojny, L ;
Durkovic, B ;
Rossmanith, W ;
Paton, D .
JOURNAL OF VIROLOGICAL METHODS, 2001, 92 (01) :19-22