Superantigens in Staphylococcus aureus isolated from prosthetic joint infection

被引：8

作者：

Kim, Choon K. ^{[1
,5
]}

Karau, Melissa J. ^{[1
]}

Greenwood-Quaintance, Kerryl E. ^{[1
]}

Tilahun, Ashenafi Y. ^{[3
]}

David, Chella S. ^{[3
]}

Mandrekar, Jayawant N. ^{[4
]}

Patel, Robin ^{[1
,2
]}

Rajagopalan, Govindarajan ^{[2
,3
]}

机构：

[1] Mayo Clin, Dept Lab Med & Pathol, Div Clin Microbiol, Rochester, MN 55905 USA

[2] Dept Med, Mayo Clin, Div Infect Dis, Rochester, MN USA

[3] Dept Immunol, Mayo Clin, Rochester, MN USA

[4] Mayo Clin, Dept Hlth Sci Res, Div Biomed Stat & Informat, Rochester, MN USA

[5] Vet Hlth Serv, Med Ctr, Div Infect Dis, Seoul, South Korea

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2015年 / 81卷 / 03期

关键词：

S; aureus; Superantigen; Prosthetic joint infection; ENTEROTOXIN GENE; T-CELLS; COMPARATIVE PREVALENCE; BIOFILM FORMATION; VANCOMYCIN; ENDOCARDITIS; RESISTANT; ANERGY; EGC; PCR;

D O I：

10.1016/j.diagmicrobio.2014.11.007

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Staphylococcus aureus is a common cause of prosthetic joint infection (PJI). The prevalence of superantigens (SAgs) among PJI-associated S. aureus is unknown. Eighty-four S. aureus isolates associated with PJI isolated between 1999 and 2006 were studied. SAg genes, sea, sob, sec, sed, see, seg, seh, sei, and tst, were assayed by PCR. Seventy-eight (92.9%) isolates carried at least 1 SAg gene studied, with 61(72.6%) harboring more than 1. seg was most commonly (70.2%), and seh was least frequently (4.8%) detected. tst-positive isolates were associated with early infection and increased erythrocyte sedimentation rate at diagnosis (P = 0.006 and P = 0.021, respectively). seg and sei were associated with methicillin resistance (P = 0.008 and P = 0.002, respectively). A majority of PJI-associated isolates studied produced biologically active SAgs in both planktonic and biofilm growth modes. SAg genes are prevalent in S. aureus causing PJI. (C) 2015 Elsevier Inc. All rights reserved.

引用

页码：201 / 207

页数：7

共 44 条

[1] Impact of Vancomycin on sarA-Mediated Biofilm Formation: Role in Persistent Endovascular Infections Due to Methicillin-Resistant Staphylococcus aureus [J].

Abdelhady, Wessam ;

Bayer, Arnold S. ;

Seidl, Kati ;

Moormeier, Derek E. ;

Bayles, Kenneth W. ;

Cheung, Ambrose ;

Yeaman, Michael R. ;

Xiong, Yan Q. .

JOURNAL OF INFECTIOUS DISEASES, 2014, 209 (08) :1231-1240

[2] Reduced Vancomycin Susceptibility in an In Vitro Catheter-Related Biofilm Model Correlates with Poor Therapeutic Outcomes in Experimental Endocarditis Due to Methicillin-Resistant Staphylococcus aureus [J].

Abdelhady, Wessam ;

Bayer, Arnold S. ;

Seidl, Kati ;

Nast, Cynthia C. ;

Kiedrowski, Megan R. ;

Horswill, Alexander R. ;

Yeaman, Michael R. ;

Xiong, Yan Q. .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2013, 57 (03) :1447-1454

[3] PCR detection of staphylococcal enterotoxin genes in Staphylococcus spp. strains isolated from meat and dairy products.: Evidence for new variants of seG and seI in S-aureus AB-8802 [J].

Blaiotta, G ;

Ercolini, D ;

Pennacchia, C ;

Fusco, V ;

Casaburi, A ;

Pepe, O ;

Villani, F .

JOURNAL OF APPLIED MICROBIOLOGY, 2004, 97 (04) :719-730

[4] Biotyping of enterotoxigenic Staphylococcus aureus by enterotoxin gene cluster (egc) polymorphism and spa typing analyses [J].

Blaiotta, Giuseppe ;

Fusco, Vincenzina ;

von Eiff, Christof ;

Villani, Francesco ;

Becker, Karsten .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2006, 72 (09) :6117-6123

[5] agr-mediated dispersal of Staphylococcus aureus biofilms [J].

Boles, Blaise R. ;

Horswill, Alexander R. .

PLOS PATHOGENS, 2008, 4 (04)

[6] agr-Genotyping and transcriptional analysis of biofilm-producing Staphylococcus aureus [J].

Cafiso, Viviana ;

Bertuccio, Taschia ;

Santagati, Maria ;

Demelio, Vanessa ;

Spina, Daniela ;

Nicoletti, Giuseppe ;

Stefani, Stefania .

FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY, 2007, 51 (01) :220-227

[7] Superantigen profiling of Staphylococcus aureus infective endocarditis isolates [J].

Chung, Jin-Won ;

Karau, Melissa J. ;

Greenwood-Quaintance, Kerryl E. ;

Ballard, Alessandro D. ;

Tilahun, Ashenafi ;

Khaleghi, Shahryar Rostamkolaei ;

David, Chella S. ;

Patel, Robin ;

Rajagopalan, Govindarajan .

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2014, 79 (02) :119-124

[8] Associations between enterotoxin gene cluster types egc1, egc2 and egc3, agr types, enterotoxin and enterotoxin-like gene profiles, and molecular typing characteristics of human nasal carriage and animal isolates of Staphylococcus aureus [J].

Collery, Mark M. ;

Smyth, Davida S. ;

Tumilty, John J. G. ;

Twohig, Jane M. ;

Smyth, Cyril J. .

JOURNAL OF MEDICAL MICROBIOLOGY, 2009, 58 (01) :13-25

[9] Clinical and molecular aspects of the pathogenesis of Staphylococcus aureus bone and joint infections [J].

Cunningham, R ;

Cockayne, A ;

Humphreys, H .

JOURNAL OF MEDICAL MICROBIOLOGY, 1996, 44 (03) :157-164

[10] Effect of Electrical Current on the Activities of Antimicrobial Agents against Pseudomonas aeruginosa, Staphylococcus aureus, and Staphylococcus epidermidis Biofilms [J].

del Pozo, Jose L. ;

Rouse, Mark S. ;

Mandrekar, Jayawant N. ;

Sampedro, Marta Fernandez ;

Steckelberg, James M. ;

Patel, Robin .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2009, 53 (01) :35-40

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