Phosphate-triggered ratiometric fluoroimmunoassay based on nanobody-alkaline phosphatase fusion for sensitive detection of 1-naphthol for the exposure assessment of pesticide carbaryl

被引：37

作者：

Chen, Zi-Jian ^{[1
]}

Wu, Hui-Ling ^{[1
]}

Shen, Yu-Dong ^{[1
]}

Wang, Hong ^{[1
]}

Zhang, Yi-Feng ^{[1
]}

Hammock, Bruce ^{[2
,3
]}

Li, Zhen-Feng ^{[2
,3
,4
]}

Luo, Lin ^{[1
]}

Lei, Hong-Tao ^{[1
]}

Xu, Zhen-Lin ^{[1
]}

机构：

[1] South China Agr Univ, Guangdong Prov Key Lab Food Qual & Safety, Guangdong Lab Lingnan Modern Agr, Guangzhou 510642, Peoples R China

[2] Univ Calif Davis, Dept Entomol, Davis, CA 95616 USA

[3] Univ Calif Davis, UCD Comprehens Canc Ctr, Davis, CA 95616 USA

[4] Guangdong Hengrui Pharmaceut Co Ltd, Guangzhou 510799, Peoples R China

来源：

JOURNAL OF HAZARDOUS MATERIALS | 2022年 / 424卷

关键词：

1-Naphthol; Carbaryl; Nanobody; Ratiometric fluoroimmunoassay; Pesticide exposure; GOLD NANOCLUSTERS; QUANTUM DOTS; HUMAN URINE; FLUORESCENCE; CHROMATOGRAPHY; NANOPARTICLES; CHLORPYRIFOS; METABOLITES; SINGLE; ENZYME;

D O I：

10.1016/j.jhazmat.2021.127411

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The excessive use of carbaryl has resulted in the risk of its exposure. In this study, we isolated six nanobodies (Nbs) from a camelid phage display library against the biomarker of carbaryl, 1-naphthol (1-NAP). Owing to its characteristics of easy genetic modifications, we produced a nanobody-alkaline phosphatase (Nb-CC4-ALP) fusion protein with good stability. A dual-emission system based ratiometric fluomimmunoassay (RFIA) for quick and highly sensitive determination of 1-NAP was developed. Silicon nanoparticles (SiNPs) was used as an internal reference and for aggregation-induced emission enhancement (AIEE) of gold nanoclusters (AuNCs), while AuNCs could be quenched by MnO2 via oxidation. In the presence of ALP, ascorbic acid phosphate (AAP) can be transformed into ascorbic acid (AA), the later can etch MnO2 to recover the fluorescence of the AuNCs. Based on optimal conditions, the proposed assay showed 220-fold sensitivity improvement in comparison with conventional monoclonal antibody-based ELISA. The recovery test of urine samples and the validation by standard HPLC-FLD demonstrated the proposed assay was an ideal tool for screening 1-NAP and provided technical support for the monitoring of carbaryl exposure.

引用

页数：10

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