What biologists want from their chloride reporters - a conversation between chemists and biologists

被引：29

作者：

Zajac, Matthew ^{[1
,2
]}

Chakraborty, Kasturi ^{[1
,2
,3
]}

Saha, Sonali ^{[4
,12
]}

Mahadevan, Vivek ^{[5
]}

Infield, Daniel T. ^{[6
]}

Accardi, Alessio ^{[7
,8
,9
]}

Qiu, Zhaozhu ^{[10
,11
]}

Krishnan, Yamuna ^{[1
,2
]}

机构：

[1] Univ Chicago, Dept Chem, 5735 S Ellis Ave, Chicago, IL 60637 USA

[2] Univ Chicago, Grossman Inst Neurosci Quantitat Biol & Human Beh, Chicago, IL 60637 USA

[3] Univ Chicago, Ben May Dept Canc Res, Chicago, IL 60637 USA

[4] Leibniz Forschungsinst Mol Pharmakol FMP, D-13125 Berlin, Germany

[5] Univ Toronto, Dept Cell & Syst Biol, Toronto, ON M5S 3G5, Canada

[6] Univ Iowa, Dept Mol Physiol & Biophys, Iowa City, IA 52242 USA

[7] Weill Cornell Med Sch, Dept Anesthesiol, New York, NY 10065 USA

[8] Weill Cornell Med Sch, Dept Physiol & Biophys, New York, NY 10065 USA

[9] Weill Cornell Med Sch, Dept Biochem, New York, NY 10065 USA

[10] Johns Hopkins Univ, Sch Med, Dept Physiol, Baltimore, MD 21218 USA

[11] Johns Hopkins Univ, Sch Med, Solomon H Snyder Dept Neurosci, Baltimore, MD 21218 USA

[12] Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Sect Cellular & Synapt Physiol, NIH, Bethesda, MD 20892 USA

来源：

JOURNAL OF CELL SCIENCE | 2020年 / 133卷 / 02期

基金：

英国惠康基金;

关键词：

Chloride; Channel; Transporter; Fluorescent reporter; probe; Neuronal signaling; Epithelial secretion; Lysosome function; GREEN FLUORESCENT PROTEIN; SENSING ION CHANNELS; CYSTIC-FIBROSIS GENE; INTRACELLULAR CHLORIDE; ANION CHANNEL; PROTON PUMP; MEMBRANE-PROTEIN; MOUSE MODEL; PH CHANGES; LYSOSOMAL ACIDIFICATION;

D O I：

10.1242/jcs.240390

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Impaired chloride transport affects diverse processes ranging from neuron excitability to water secretion, which underlie epilepsy and cystic fibrosis, respectively. The ability to image chloride fluxes with fluorescent probes has been essential for the investigation of the roles of chloride channels and transporters in health and disease. Therefore, developing effective fluorescent chloride reporters is critical to characterizing chloride transporters and discovering new ones. However, each chloride channel or transporter has a unique functional context that demands a suite of chloride probes with appropriate sensing characteristics. This Review seeks to juxtapose the biology of chloride transport with the chemistries underlying chloride sensors by exploring the various biological roles of chloride and highlighting the insights delivered by studies using chloride reporters. We then delineate the evolution of small-molecule sensors and genetically encoded chloride reporters. Finally, we analyze discussions with chloride biologists to identify the advantages and limitations of sensors in each biological context, as well as to recognize the key design challenges that must be overcome for developing the next generation of chloride sensors.

引用

页数：13

共 182 条

[91] Ratiometric fluorescence imaging for distinguishing chloride concentration between normal and ischemic ventricular myocytes [J].