Promotion of cutaneous wound healing by local application of mesenchymal stem cells derived from human umbilical cord blood

被引：106

作者：

Luo, Gaoxing ^{[1
]}

Cheng, Wenguang ^{[1
]}

He, Weifeng ^{[1
]}

Wang, Xiaojuan ^{[1
]}

Tan, Jiangling ^{[1
]}

Fitzgerald, Mark ^{[2
]}

Li, Xianchang ^{[3
]}

Wu, Jun ^{[1
]}

机构：

[1] Third Mil Med Univ, Southwestern Hosp, Burn Res Inst, State Key Lab Trauma Burn & Combined Injury, Chongqing 400038, Peoples R China

[2] Alfred Hosp, Trauma Serv, Melbourne, Vic, Australia

[3] Harvard Univ, Beth Israel Deaconess Med Ctr, Sch Med, Transplant Res Ctr, Boston, MA 02215 USA

来源：

WOUND REPAIR AND REGENERATION | 2010年 / 18卷 / 05期

基金：

中国国家自然科学基金;

关键词：

MARROW STROMAL CELLS; BONE-MARROW; ADIPOSE-TISSUE; IN-VIVO; DISEASE; DIFFERENTIATION; REGENERATION; SKIN; CONTRIBUTE; INDUCTION;

D O I：

10.1111/j.1524-475X.2010.00616.x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

This study aimed to determine whether mesenchymal stem cells (MSCs) derived from umbilical cord blood (UCB) would promote cutaneous wound healing. MSCs from human UCB were isolated and identified. The characteristics of the isolated MSCs' growth and proliferation were assayed in vitro. The MSCs labeled with 5-bromodeoxyuridine (BrdU) were applied on fresh cutaneous mice wounds. The healing rates were surveyed. The distribution and the differentiation into keratinocytes of the labeled MSCs in the wound tissue were checked by immunohistochemistry staining. The isolated MSCs could grow and proliferate well in vitro. The isolated MSCs from UCB could be labeled by 5-bromodeoxyuridine successfully. The MSCs derived from UCB could enhance the healing of mice skin defect wounds, and it was found that the implanted MSCs could differentiate into keratinocyte in the wound tissue. It was demonstrated that MSCs from UCB can be isolated and proliferated successfully. The local administration of MSCs derived from UCB improves skin defect wound healing in mice.

引用

页码：506 / 513

页数：8

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