Cutting edge:: T lymphocyte activation by repeated immunological synapse formation and intermittent signaling

被引:87
作者
Faroudi, M [1 ]
Zaru, R [1 ]
Paulet, P [1 ]
Müller, S [1 ]
Valitutti, S [1 ]
机构
[1] Inst Claude Preval, Lymphocyte Interact Grp, INSERM, U563, Toulouse, France
关键词
D O I
10.4049/jimmunol.171.3.1128
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The activation of biological T cell responses requires prolonged contact with APCs and sustained signaling. We investigated whether signaling must be uninterrupted to commit T cells to cytokine production or whether T cell activation may also result from summation of interrupted signals. Upon periodic addition and removal of a src kinase inhibitor, human CD4(+) T cells destroyed and reformed immunological synapses while aborting and restarting signal transduction. Remarkably, under these conditions, T cells were eventually activated to IFN-gamma production and the amount of IFN-gamma produced was directly related to the total signaling time despite the repeated interruptions. Our results illustrate that T cell activation does not require a stable immunological synapse and can be achieved by interrupted signaling. It is implied that T cells can add activation signals, possibly collected on multiple APCs.
引用
收藏
页码:1128 / 1132
页数:5
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