GERMLINE MODIFICATION USING MOUSE SPERMATOGONIAL STEM CELLS

被引：23
|
作者：
Kanatsu-Shinohara, Mito [1 ]
Shinohara, Takashi [1 ]
机构：
[1] Kyoto Univ, Dept Mol Genet, Grad Sch Med, Kyoto, Japan
来源：
METHODS IN ENZYMOLOGY, VOL 477: GUIDE TO TECHNIQUES IN MOUSE DEVELOPMENT, PART B: MOUSE MOLECULAR GENETICS, SECOND EDITION | 2010年 / 477卷
关键词：
LONG-TERM PROLIFERATION; MEDIATED GENE DELIVERY; KNOCKOUT MICE; IN-VITRO; TRANSPLANTATION; SPERMATOGENESIS; TRANSMISSION; TRANSDUCTION; GENERATION; GROWTH;
D O I：
10.1016/S0076-6879(10)77002-6
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Spermatogonial stem cells (SSCs) in the testes are a new target for germline modification. With the development of an in vitro culture system and spermatogonial transplantation technique, SSCs can now be manipulated and used as an alternative to embryonic stem cells for knockout mice production. The genetic and epigenetic stability of SSCs provide new possibilities for the application of germline mutagenesis in a wide range of animals.
引用
收藏
页码：17 / 36
页数：20
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