Chemical Characterization and Bioactivity of Extracts from Thymus mastichina: A Thymus with a Distinct Salvianolic Acid Composition

被引:27
|
作者
Taghouti, Meriem [1 ,2 ]
Martins-Gomes, Carlos [1 ,2 ]
Schaefer, Judith [3 ]
Santos, Joao A. [1 ,4 ]
Bunzel, Mirko [3 ]
Nunes, Fernando M. [2 ,5 ]
Silva, Amelia M. [1 ,6 ]
机构
[1] Univ Tras Os Montes & Alto Douro UTAD, Ctr Res & Technol Agroenvironm & Biol Sci CITAB, P-5001801 Vila Real, Portugal
[2] Univ Tras Os Montes & Alto Douro UTAD, Chem Res Ctr Vila Real CQ VR, Food & Wine Chem Lab, P-5001801 Vila Real, Portugal
[3] KIT, Inst Appl Biosci, Dept Food Chem & Phytochem, Adenauerring 20a,Bldg 50-41, D-76131 Karlsruhe, Germany
[4] UTAD, Dept Phys, Sch Sci & Technol, P-5001801 Vila Real, Portugal
[5] UTAD, Dept Chem, Sch Life Sci & Environm, P-5001801 Vila Real, Portugal
[6] Univ Tras Os Montes & Alto Douro UTAD, Sch Life Sci & Environm, Dept Biol & Environm, P-5001801 Vila Real, Portugal
关键词
Thymus mastichina; phenolic profiling; aqueous decoction; hydroethanolic extract; salvianolic acid A isomer; salvianolic acid isomer B; E isomer; anti-proliferative activity; radical scavenging activity; antioxidant; ANTIOXIDANT ACTIVITY; PHENOLIC-COMPOUNDS; POLYPHENOLS; NANOPARTICLES; CACO-2; CANCER; MENTHA;
D O I
10.3390/antiox9010034
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Thymus mastichina, also called mastic thyme or Spanish marjoram, is endemic to the Iberian Peninsula, where it is widely used in folk medicine especially for treating digestive and respiratory systems disorders, and as a condiment to season olives. This work describes for the first time the detailed phenolic composition of exhaustive hydroethanolic extracts and aqueous decoctions of Thymus mastichina. Unlike other species of the Thymus genera, Thymus mastichina extracts contain high amounts of salvianolic acid derivatives, with salvianolic acid A isomer being the main derivative. This isomer was identified in extracts from Thymus mastichina for the first time. Also, an undescribed salvianolic acid derivative in Thymus mastichina was identified and its structure was tentatively described. Extracts from Thymus mastichina showed significant scavenging activity of 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical cation, hydroxyl, and nitric oxide radicals. The anti-proliferative effect of both T. mastichina extracts were tested against Caco-2 and HepG2 cells; the hydroethanolic extract showed a high anti-proliferative activity against Caco-2 cells compared to HepG2 cells (at 24 h exposure, the concentration that inhibits 50% of proliferation, IC50, was 71.18 +/- 1.05 mu g/mL and 264.60 +/- 11.78 mu g/mL for Caco-2 and HepG2, respectively). Thus, these results make this species a promising candidate for further investigation of its anti-tumoral potential. Therefore, Thymus mastichina can be potentially used as a functional food (used as a decoction or herbal tea) or as a source of bioactive ingredients with antioxidant and anti-proliferative properties.
引用
收藏
页数:18
相关论文
共 50 条
  • [31] Chemical characterization and bioactive properties of decoctions and hydroethanolic extracts of Thymus carnosus Boiss
    Martins-Gomes, Carlos
    Taghouti, Meriem
    Schaefer, Judith
    Bunzel, Mirko
    Silva, Amelia M.
    Nunes, Fernando M.
    JOURNAL OF FUNCTIONAL FOODS, 2018, 43 : 154 - 164
  • [32] Chemical Composition and Antifungal Activities of the Essential Oils of Thymus Species (Thymus pectinatus, Thymus convolutus, Thymus vulgaris) Against Plant Pathogens
    Aksit, Huseyin
    Bayar, Yusuf
    Simsek, Samed
    Ulutas, Yakup
    JOURNAL OF ESSENTIAL OIL BEARING PLANTS, 2022, 25 (01) : 200 - 207
  • [33] Effect of substrate on the essential oils composition of Thymus mastichina (L.) L. subsp mastichina collected in Sesimbra region (Portugal)
    Miguel, G
    Guerrero, C
    Rodrigues, H
    Brito, J
    Venâncio, F
    Tavares, R
    Duarte, F
    NATURAL PRODUCTS IN THE NEW MILLENNIUM: PROSPECTS AND INDUSTRIAL APPLICATION, 2002, 47 : 143 - 148
  • [34] AMINO ACID COMPOSITION OF THYMUS NUCLEOHISTONE
    MORRIS, MD
    HARPER, HA
    PROCEEDINGS OF THE SOCIETY FOR EXPERIMENTAL BIOLOGY AND MEDICINE, 1953, 82 (03): : 482 - 484
  • [35] Chemical composition of essential oil from Thymus vulgaris hybrid
    Mirza, M
    Baher, ZF
    JOURNAL OF ESSENTIAL OIL RESEARCH, 2003, 15 (06) : 404 - 405
  • [36] Composition and characteristics of distinct macrophage subpopulations in the mouse thymus
    Liu, Lan-Tao
    Lang, Zhi-Fang
    Li, Ying
    Zhu, Yong-Jie
    Zhang, Jiu-Tao
    Guo, Shang-Fu
    Wang, Ji-Xi
    Wang, Hong-Wei
    Xu, Yu-Dong
    MOLECULAR MEDICINE REPORTS, 2013, 7 (06) : 1850 - 1854
  • [37] ISOLATION AND CHARACTERIZATION OF A DNA HELICASE FROM CYTOSOLIC EXTRACTS OF CALF THYMUS
    ZHANG, SS
    GROSSE, F
    CHROMOSOMA, 1992, 102 (01) : S100 - S106
  • [38] Chemical characterization and antibacterial activity of Thymus moroderi and Thymus piperella essential oils, two Thymus endemic species from southeast of Spain
    Ruiz-Navajas, Y.
    Viuda-Martos, M.
    Sendra, E.
    Perez-Alvarez, J. A.
    Fernandez-Lopez, J.
    FOOD CONTROL, 2012, 27 (02) : 294 - 299
  • [39] Chemical composition of Thymus punctulosus essential oil
    L. I. Alekseeva
    A. G. Bystrushkin
    I. V. Gruzdev
    Chemistry of Natural Compounds, 2010, 46 : 491 - 492