Aptamer-Functionalized DNA-Silver Nanocluster Nanofilm for Visual Detection and Elimination of Bacteria

被引：968

作者：

Yang, Min ^{[1
]}

Chen, Xu ^{[2
]}

Zhu, Longjiao ^{[1
]}

Lin, Shenghao ^{[2
]}

Li, Chenwei ^{[2
]}

Li, Xiangyang ^{[3
]}

Huang, Kunlun ^{[2
]}

Xu, Wentao ^{[1
]}

机构：

[1] China Agr Univ, Dept Nutr & Hlth, Key Lab Precis Nutr & Food Qual, Beijing 100083, Peoples R China

[2] China Agr Univ, Key Lab Safety Assessment Genetically Modified Or, Coll Food Sci & Nutr Engn, Beijing 100083, Peoples R China

[3] Beijing Univ Agr, Beijing Lab Food Qual & Safety, Beijing Key Lab Agr Prod Detect & Control Spoilag, Fac Food Sci & Engn, Beijing 102206, Peoples R China

来源：

ACS APPLIED MATERIALS & INTERFACES | 2021年 / 13卷 / 32期

基金：

中国国家自然科学基金;

关键词：

aptamers; DNA-templated silver nanoclusters; antibacterial; fluorescent; nanoflim; FLUORESCENT; NANOPARTICLES; PROBES; IONS;

D O I：

10.1021/acsami.1c05751

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

As a new type of nanomaterial, DNA-templated silver nanoclusters (DNA-AgNCs) have been widely studied because of their fluorescence and antibacterial properties. In this study, we combined the DNA-AgNCs with aptamers of bacteria to achieve a novel approach for the visual detection and effective elimination of bacteria. The aptamers of Staphylococcus aureus (S. aureus) were linked to G-rich sequences to achieve fluorescence enhancement when approaching the DNA-AgNCs. The capture of aptamers not only realized the visual monitoring of bacteria but also promoted the antibacterial effects. Additionally, a fluorescent nanofilm with excellent selectivity and antibacterial activity in the detection and elimination of S. aureus was developed based on the DNA-AgNCs. These aptamer-functionalized DNA-AgNCs show significant potential for many applications in food packaging and biomedical engineering.

引用

页码：38647 / 38655

页数：9

共 43 条

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