Effects of the thyroid hormone responsive spot 14α gene on chicken growth and fat traits

被引：18

作者：

Hirwa, C. d'Andre ^{[1
]}

Yan, W. ^{[1
]}

Wallace, P. ^{[1
]}

Nie, Q. ^{[1
]}

Luo, C. ^{[1
]}

Li, H. ^{[1
]}

Shen, X. ^{[1
]}

Sun, L. ^{[1
]}

Tang, J. ^{[1
]}

Li, W. ^{[1
]}

Zhu, X. ^{[1
]}

Yang, G. ^{[1
]}

Zhang, X. ^{[1
]}

机构：

[1] S China Agr Univ, Dept Anim Genet Breeding & Reprod, Coll Anim Sci, Guangzhou 510642, Guangdong, Peoples R China

来源：

POULTRY SCIENCE | 2010年 / 89卷 / 09期

基金：

国家高技术研究发展计划(863计划);

关键词：

thyroid hormone responsive spot 14 alpha; growth; fat deposition; chicken; LIPOPROTEIN CHOLESTEROL; EXPRESSION; PROTEIN; POLYMORPHISMS; CARBOHYDRATE; LIPOGENESIS; GLUCOSE;

D O I：

10.3382/ps.2009-00582

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

The thyroid hormone responsive spot 14 alpha (THRSP alpha) gene plays important roles in chicken growth and fat deposition. The aim of this study was to identify new variations in the gene to determine their effects on growth and fat traits in chicken and to observe the effects of the THRSP alpha gene on chicken lipid profile and lipoprotein and glucose and triiodothyronine effects on the THRSP alpha expression in liver and fat cells. Two new variations, namely A197835978G and G197836086A, and a reported 9-bp insertion-deletion (indel) of the THRSP alpha gene were genotyped by single-stranded conformational polymorphism in a Xinghua x White Recessive Rock F-2 full-sib resource population. The results showed that the A197835978G was significantly associated with hatch weight and BW at 28 d of age and breast muscle weight at 90 d of age in chickens (P < 0.05). The G197836086A was significantly associated with cingular fat width (P = 0.0349) and breast muscle crude fat content (P = 0.0349). The indel was significantly associated with abdominal fat weight (P = 0.0445). The above new THRSP alpha polymorphisms were also significantly associated with the total cholesterol and low-density lipoprotein, in which the THRSP alpha GA/AG genotype was associated with lipid and lipoprotein and the THRSP alpha BB indel genotype was significantly associated with liver weight in chicken breeds. The mRNA expression analysis in vivo and in vitro culture studies suggested that the THRSP alpha gene is more responsive to glucose than triiodothyronine. In conclusion, the 3 variations of the chicken THRSP alpha gene were associated with both growth and fat traits in this study. Such effects of the THRSP alpha gene were further supported from the data of observations in association analysis of the gene with phenotypic records and plasma lipid profiles, in the THRSP alpha gene expression in chicken development, and in vivo and in vitro cell culture observation of liver and abdominal fat tissues.

引用

页码：1981 / 1991

页数：11

共 25 条

[1] The Thrsp null mouse (Thrsptm1cnm) and diet-induced obesity [J].

Anderson, Grant W. ;

Zhu, Qihong ;

Metkowski, Jennifer ;

Stack, Mary Jo ;

Gopinath, Sunil ;

Mariash, Cary N. .

MOLECULAR AND CELLULAR ENDOCRINOLOGY, 2009, 302 (01) :99-107

[2]

[Anonymous], 1989, SAS STAT US GUID VER

[3] ''Spot 14'' protein functions at the pretranslational level in the regulation of hepatic metabolism by thyroid hormone and glucose [J].

Brown, SB ;

Maloney, M ;

Kinlaw, WB .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (04) :2163-2166

[4] Association of spotl4α gene polymorphisms with body weight in the chicken [J].

Cao, Z. P. ;

Wang, S. Z. ;

Wang, Q. G. ;

Wang, Y. X. ;

Li, H. .

POULTRY SCIENCE, 2007, 86 (09) :1873-1880

[5]

Cogburn L. A., 2003, Poultry genetics, breeding and biotechnology, P629, DOI 10.1079/9780851996608.0629

[6] Functional genomics in chickens: development of integrated-systems microarrays for transcriptional profiling and discovery of regulatory pathways [J].

Cogburn, LA ;

Wang, X ;

Carre, W ;

Rejto, L ;

Aggrey, SE ;

Duclos, MJ ;

Simon, J ;

Porter, TE .

COMPARATIVE AND FUNCTIONAL GENOMICS, 2004, 5 (03) :253-261

[7]

Cogburn LA, 2000, POULT SCI S1, V79, P72

[8]

Council N.R., 1994, Nutrient Requirements of Poultry

[9] New perspectives in the regulation of hepatic glycolytic and lipogenic genes by insulin and glucose:: a role for the transcription factor sterol regulatory element binding protein-1c [J].

Foufelle, F ;

Ferré, P .

BIOCHEMICAL JOURNAL, 2002, 366 (02) :377-391

[10] ISOLATION AND LONG-TERM MAINTENANCE OF DIFFERENTIATED ADULT CHICKEN HEPATOCYTES IN PRIMARY CULTURE [J].

FRASLIN, JM ;

TOUQUETTE, L ;

DOUAIRE, M ;

MENEZO, Y ;

GUILLEMOT, JC ;

MALLARD, J .

IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL, 1992, 28A (9-10) :615-620

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