Functionally heterogeneous human satellite cells identified by single cell RNA sequencing

被引：90

作者：

Barruet, Emilie ^{[1
,2
]}

Garcia, Steven M. ^{[1
,2
]}

Striedinger, Katharine ^{[1
,2
]}

Wu, Jake ^{[1
,2
]}

Lee, Solomon ^{[1
,2
]}

Byrnes, Lauren ^{[3
]}

Wong, Alvin ^{[1
,2
]}

Sun Xuefeng ^{[4
]}

Tamaki, Stanley ^{[1
,2
]}

Brack, Andrew S. ^{[4
]}

Pomerantz, Jason H. ^{[1
,2
]}

机构：

[1] Univ Calif San Francisco, Program Craniofacial Biol, Div Plast & Reconstruct Surg, Dept Surg,Eli & Edythe Broad Ctr Regenerat Med, San Francisco, CA 94110 USA

[2] Univ Calif San Francisco, Eli & Edythe Broad Ctr Regenerat Med, Div Plast & Reconstruct Surg, Dept Orofacial Sci,Program Craniofacial Biol, San Francisco, CA 94110 USA

[3] Univ Calif San Francisco, San Francisco, CA 94143 USA

[4] Univ Calif San Francisco, Eli & Edythe Broad Ctr Regenerat Med, Dept Orthoped Surg, San Francisco, CA 94143 USA

来源：

ELIFE | 2020年 / 9卷

基金：

美国国家卫生研究院;

关键词：

STEM-CELLS; SELF-RENEWAL; REVERSIBLE QUIESCENCE; DNA-DAMAGE; MUSCLE; CAVEOLIN-1; EXPRESSION; TRANSPLANTATION; INFLAMMATION; ACTIVATION;

D O I：

10.7554/eLife.51576

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Although heterogeneity is recognized within the murine satellite cell pool, a comprehensive understanding of distinct subpopulations and their functional relevance in human satellite cells is lacking. We used a combination of single cell RNA sequencing and flow cytometry to identify, distinguish, and physically separate novel subpopulations of human PAX7+ satellite cells (Hu-MuSCs) from normal muscles. We found that, although relatively homogeneous compared to activated satellite cells and committed progenitors, the Hu-MuSC pool contains clusters of transcriptionally distinct cells with consistency across human individuals. New surface marker combinations were enriched in transcriptional subclusters, including a subpopulation of Hu-MuSCs marked by CXCR4/CD29/CD56/CAV1 (CAV1+). In vitro, CAV1+ Hu-MuSCs are morphologically distinct, and characterized by resistance to activation compared to CAV1- Hu-MuSCs. In vivo, CAV1 + Hu-MuSCs demonstrated increased engraftment after transplantation. Our findings provide a comprehensive transcriptional view of normal Hu-MuSCs and describe new heterogeneity, enabling separation of functionally distinct human satellite cell subpopulations.

引用

页数：27

共 107 条

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