triSPIM: light sheet microscopy with isotropic super-resolution

被引:15
作者
Manton, James D. [1 ]
Rees, Eric J. [1 ]
机构
[1] Univ Cambridge, Dept Chem Engn & Biotechnol, Cambridge CB2 3RA, England
基金
英国工程与自然科学研究理事会;
关键词
PLANE ILLUMINATION MICROSCOPY; AXIAL RESOLUTION; LIMIT; BEAM;
D O I
10.1364/OL.41.004170
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
We propose a three-objective light sheet microscopy geometry which, through a combination of skewed lattice light sheet excitation through two objectives and the computational fusion of images taken from two separate lens pairings, would allow for isotropic super-resolution in mesoscopic samples. We also show that simultaneous coherent excitation through two excitation objectives could further substantially increase resolution. Simulations demonstrate that our design could achieve a resolution of 120 nm for EGFP imaging while minimizing photodamage. Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License.
引用
收藏
页码:4170 / 4173
页数:4
相关论文
共 18 条
[1]   Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution [J].
Chen, Bi-Chang ;
Legant, Wesley R. ;
Wang, Kai ;
Shao, Lin ;
Milkie, Daniel E. ;
Davidson, Michael W. ;
Janetopoulos, Chris ;
Wu, Xufeng S. ;
Hammer, John A., III ;
Liu, Zhe ;
English, Brian P. ;
Mimori-Kiyosue, Yuko ;
Romero, Daniel P. ;
Ritter, Alex T. ;
Lippincott-Schwartz, Jennifer ;
Fritz-Laylin, Lillian ;
Mullins, R. Dyche ;
Mitchell, Diana M. ;
Bembenek, Joshua N. ;
Reymann, Anne-Cecile ;
Boehme, Ralph ;
Grill, Stephan W. ;
Wang, Jennifer T. ;
Seydoux, Geraldine ;
Tulu, U. Serdar ;
Kiehart, Daniel P. ;
Betzig, Eric .
SCIENCE, 2014, 346 (6208) :439-+
[2]   STED-SPIM: Stimulated Emission Depletion Improves Sheet Illumination Microscopy Resolution [J].
Friedrich, Mike ;
Gan, Qiang ;
Ermolayev, Vladimir ;
Harms, Gregory S. .
BIOPHYSICAL JOURNAL, 2011, 100 (08) :L43-L45
[3]  
Gustafsson MGL, 1999, J MICROSC-OXFORD, V195, P10, DOI 10.1046/j.1365-2818.1999.00576.x
[4]  
GUSTAFSSON MGL, 1995, P SOC PHOTO-OPT INS, V2412, P147, DOI 10.1117/12.205334
[5]   Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy [J].
Gustafsson, MGL .
JOURNAL OF MICROSCOPY, 2000, 198 (02) :82-87
[6]   Breaking the diffraction limit of light-sheet fluorescence microscopy by RESOLFT [J].
Hoyer, Patrick ;
de Medeiros, Gustavo ;
Balazs, Balint ;
Norlin, Nils ;
Besir, Christina ;
Hanne, Janina ;
Kraeusslich, Hans-Georg ;
Engelhardt, Johann ;
Sahl, Steffen J. ;
Hell, Stefan W. ;
Hufnagel, Lars .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2016, 113 (13) :3442-3446
[7]   Interferometric imaging with three objectives [J].
Huelsnitz, Thomas ;
Kner, Peter .
JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION, 2016, 33 (02) :179-191
[8]   Optical sectioning deep inside live embryos by selective plane illumination microscopy [J].
Huisken, J ;
Swoger, J ;
Del Bene, F ;
Wittbrodt, J ;
Stelzer, EHK .
SCIENCE, 2004, 305 (5686) :1007-1009
[9]   Even fluorescence excitation by multidirectional selective plane illumination microscopy (mSPIM) [J].
Huisken, Jan ;
Stainier, Didier Y. R. .
OPTICS LETTERS, 2007, 32 (17) :2608-2610
[10]  
Keller PJ, 2010, NAT METHODS, V7, P637, DOI [10.1038/NMETH.1476, 10.1038/nmeth.1476]