Evaluation of Apoptosis and Autophagy Activities in the Brain of Dogs Naturally Infected with Canine Distemper Virus Based on Changes in Apoptotic and Autophagic Markers

被引:1
|
作者
Degirmencay, Sukru [1 ]
Ozdemir, Selcuk [2 ]
机构
[1] Ataturk Univ, Dept Internal Med, Fac Vet Med, TR-25240 Erzurum, Turkey
[2] Ataturk Univ, Fac Vet Med, Dept Genet, TR-25240 Erzurum, Turkey
来源
ACTA VETERINARIA-BEOGRAD | 2022年 / 72卷 / 02期
关键词
apoptosis; autophagy; canine distemper; real-time PCR; ENCEPHALOMYELITIS; MORBILLIVIRUS; MECHANISMS; EXPRESSION; MULTIPLE; FUSION; DEATH; LC3;
D O I
10.2478/acve-2022-0016
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
This study investig ated the activation of apoptosis and autophagy in CDV infected brain tissues of dogs with acute neurological signs, by determining Cas-3, Cas-8, Cas-9, Bax, Bcl-2, LC3B, and Beclin-1 expression with real-time PCR. The expression levels of Beclin-1 and LC3B, autophagy markers, were significantly up-regulated in comparison with the control group (p < 0.001). The expression levels of apoptotic markers Cas-3, Cas-8, Cas-9 and Bax were slightly up-regulated, but Bcl-2 was slightly down-regulated in contrast to the control group (p < 0.05). Therefore, the autophagy markers were more activated than apoptotic markers in dogs with acute neurological signs. In conclusion, autophagy takes part in the pathogenesis of demyelination in canine distemper. Knowing this may be helpful to create new therapeutic strategies, such as new effective antiviral medicines.
引用
收藏
页码:195 / 206
页数:12
相关论文
共 17 条
  • [1] Apoptotic investigation of brain tissue cells in dogs naturally infected by canine distemper virus
    Pan, Yaoqian
    Wang, Shuai
    Li, Peng
    Yue, Feng
    Zhang, Yanfang
    Pan, Bo
    Liu, Xingyou
    VIROLOGY JOURNAL, 2021, 18 (01)
  • [2] Apoptotic investigation of brain tissue cells in dogs naturally infected by canine distemper virus
    Yaoqian Pan
    Shuai Wang
    Peng Li
    Feng Yue
    Yanfang Zhang
    Bo Pan
    Xingyou Liu
    Virology Journal, 18
  • [3] Pathologic and immunohistochemistry findings in dogs naturally infected by canine distemper virus
    Sonne, Luciana
    Oliveira, Eduardo C.
    Pescador, Caroline A.
    Santos, Adriana S.
    Pavarini, Saulo P.
    Carissimi, Andre S.
    Driemeier, David
    PESQUISA VETERINARIA BRASILEIRA, 2009, 29 (02): : 143 - 149
  • [4] Rapid molecular detection and isolation of canine distemper virus in naturally infected dogs
    Saltik, Hasbi Sait
    Kale, Mehmet
    ANKARA UNIVERSITESI VETERINER FAKULTESI DERGISI, 2023, 70 (01): : 49 - 56
  • [5] Comparative evaluation of clinical samples from naturally infected dogs for early detection of canine distemper virus
    Shabbir, Muhammad Zubair
    Rabbani, Masood
    Ahmad, Arfan
    Ahmed, Ali
    Muhammad, Khushi
    Anwar, Ikram
    TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES, 2010, 34 (06): : 547 - 552
  • [6] Prolonged persistence of canine distemper virus RNA, and virus isolation in naturally infected shelter dogs
    Allen, Carolyn
    Ellis, Alexandre
    Liang, Ruibin
    Lim, Ailam
    Newbury, Sandra
    PLOS ONE, 2023, 18 (01):
  • [7] IMMUNOHISTOCHEMICAL ANALYSIS OF THE LYMPHOID ORGANS OF DOGS NATURALLY INFECTED WITH CANINE-DISTEMPER VIRUS
    IWATSUKI, K
    OKITA, M
    OCHIKUBO, F
    GEMMA, T
    SHIN, YS
    MIYASHITA, N
    MIKAMI, T
    KAI, C
    JOURNAL OF COMPARATIVE PATHOLOGY, 1995, 113 (02) : 185 - 190
  • [8] The side effects of ribavirin, prednisone and DMSO in dogs naturally infected by canine distemper virus
    Mangia, Simone H.
    Moraes, Livia F.
    Takahira, Regina K.
    Motta, Rodrigo G.
    Franco, Marilia M. J.
    Megid, Jane
    Silva, Aristeu V.
    Paes, Antonio C.
    PESQUISA VETERINARIA BRASILEIRA, 2014, 34 (05): : 449 - 454
  • [9] Prolonged Visual Evoked Potential Latencies in Dogs Naturally Infected with Canine Distemper Virus
    Gutierrez, Mary
    Delucchi, Luis
    Bielli, Alejandro
    Verdes, Jose Manuel
    VIRUSES-BASEL, 2024, 16 (11):
  • [10] Inducible nitric oxide synthase expression in cerebellum of dogs naturally infected with canine distemper virus
    Yarim, Murat
    Karayigit, M. Onder
    Gacar, Ayhan
    Kabak, Yonca B.
    Gulbahar, M. Yavuz
    Guvenc, Tolga
    Sozmen, Mahmut
    ANKARA UNIVERSITESI VETERINER FAKULTESI DERGISI, 2015, 62 (03): : 189 - 196