MiR-138-5p targeting LIMK1 suppresses breast cancer cell proliferation and motility

被引：8

作者：

Li, Dengfeng ^{[1
]}

Song, Hongming ^{[1
]}

Wu, Tianqi ^{[1
]}

Xie, Dan ^{[1
]}

Hu, Jiashu ^{[1
]}

Zhao, Junyong ^{[1
]}

Fang, Lin ^{[1
]}

机构：

[1] Tongji Univ, Sch Med, Dept Thyroid & Breast, Gen Surg,Shanghai Peoples Hosp 10,Jingan Area, 301 Yanchang Middle Rd, Shanghai 200072, Peoples R China

来源：

RSC ADVANCES | 2017年 / 7卷 / 82期

基金：

中国国家自然科学基金;

关键词：

ARYL UREA DERIVATIVES; METASTASIS; GROWTH; INHIBITORS; INVASION; IDENTIFICATION; PROGRESSION; DISCOVERY; THERAPY; MARKERS;

D O I：

10.1039/c7ra09042k

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Breast cancer is the most frequently diagnosed female cancer. LIM domain kinase 1 (LIMK1) was reported to increase breast cancer progressive biological behavior and was associated with clinical breast cancer outcomes. MiRNAs are a kind of small non-coding RNA that binds the target mRNAs, leading to protein downregulation. The aim of this study was to find whether miR-138-5p could target LIMK1 in breast cancer to suppress breast cancer biological behavior. The TCGA database showed that LIMK1 was highly expressed in breast cancer. The dual-luciferase report showed that LIMK1 was a target gene of miR-138-5p. Over-expression of miR-138-5p could significantly reduce expression of LIMK1 in mRNA and the protein level. Colony formation and MTT assay showed that over-expression of miR-138-5p could suppress proliferation of breast cancer cells. Wound-healing assay and transwell assay showed that over-expression of miR-138-5p could suppress migration and invasion of breast cancer cells. In addition, over-expression of miR-138-5p could arrest breast cancer cells into the G0/G1 phase by down-regulating expression of CDK4, 6/Cyclin D1 and CDK2/Cyclin E1 compounds. In conclusion, our study showed that miR-138-5p could target LIMK1 and suppress the progressive biological behavior of breast cancer cells. Mi-138-5p targeting LIMK1 may be a new potential treatment for breast cancer.

引用

页码：52030 / 52038

页数：9

共 42 条

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