Identification and fine mapping of molecular markers closely linked to fruit spines size ss gene in cucumber (Cucumis sativus L.)

被引：9

作者：

Zhang Weiwei ^{[1
,2
]}

Chen Yue ^{[2
]}

Zhou Peng ^{[1
]}

Bao Wenmin ^{[1
]}

Yang Xuqin ^{[3
,4
]}

Xu Taibai ^{[1
]}

She Weiwei ^{[1
]}

Xu Liqin ^{[1
]}

Yu Pinggao ^{[1
]}

Pan Junsong ^{[2
]}

机构：

[1] Shanghai Vocat Coll Agr & Forestry, Dept Plant Sci & Technol, Zhongshaner Rd, Shanghai 201699, Peoples R China

[2] Shanghai Jiao Tong Univ, Sch Agr & Biol, Dongchuan Rd, Shanghai 200240, Peoples R China

[3] Jiangsu Normal Univ, Key Lab Biotechnol Med Plants Jiangsu Prov, Xuzhou 221116, Jiangsu, Peoples R China

[4] Jiangsu Normal Univ, Sch Life Sci, Xuzhou 221116, Jiangsu, Peoples R China

来源：

EUPHYTICA | 2018年 / 214卷 / 11期

基金：

中国国家自然科学基金;

关键词：

Cucumber; Fruit external quality; Fruit spine; Mapping; MAS; QTL ANALYSIS; TRAITS; CONSTRUCTION; SKIN; MAP;

D O I：

10.1007/s10681-018-2251-y

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Fruit spine size is one of the importantly external quality traits effected the economic value of cucumber fruit. Morphological-cytological observation of the fruit spine size phenotype indicated that large spine formation arises from an increasing of spiny pedestal cell number caused by cell division, and best periods to accurately score fruit spine size trait was 4th day before flowering to 7th day after flowering according the continuous observation. Genetic analysis showed that a single dominant gene determined the fruit spine size trait in cucumber. BC1 population (189 individuals) of two inbred lines (large spine PI197088 and small spine SA0422) was used for primary mapping of the SS/ss locus with 7 markers covering an interval of 37.1cM. An F-2 segregating population of 1032 individuals constructed from the same two parents (PI197088 and SA0422) was used to fine mapping of the SS/ss locus. Six new markers linked to the gene were successfully screened for construction of a fine linkage map, in which the SS/ss locus was located in the region flanked by marker SE1 (3 recombinants) and SSR43 (2 recombinants) with a 189kb physical distance. Markers from this study will be valuable for candidate gene cloning and marker-assisted selection for cucumber breeding.

引用

页数：10