β1 integrin expression on endothelial cells is required for angiogenesis but not for vasculogenesis

被引：67

作者：

Tanjore, Harikrishna ^{[1
]}

Zeisberg, Elisabeth M. ^{[1
]}

Gerami-Naini, Behzad ^{[1
]}

Kalluri, Raghu ^{[2
,3
]}

机构：

[1] Harvard Univ, Sch Med, Beth Israel Deaconess Med Ctr, Dept Med,Div Matrix Biol, Boston, MA 02215 USA

[2] Harvard Univ, Sch Med, Dept Biol Chem & Mol Pharmacol, Boston, MA 02115 USA

[3] Harvard MIT Div Hlth Sci & Technol, Boston, MA USA

来源：

DEVELOPMENTAL DYNAMICS | 2008年 / 237卷 / 01期

关键词：

beta; 1; integrin; Tie-2; Cre; endothelial cells; angiogenesis;

D O I：

10.1002/dvdy.21385

中图分类号：

R602 [外科病理学、解剖学]; R32 [人体形态学];

学科分类号：

100101 ;

摘要：

Integrins are a family of cell adhesion receptors that are involved in cell-matrix and cell-cell communications. They facilitate cell proliferation, migration, and survival. Using the Cre-Lox system, we deleted beta 1 integrin on Tie2-positive (Tie2-cre beta 1 Int(fl/fl)) vascular endothelial cells. Deletion of beta 1 integrin on vascular endothelial cells results in embryonic lethality. Blood vessel defects are encountered in the Tie2-Cre beta 1 Int(fl/fl) embryos at embryonic age (E9.5), and embryos die before reaching E10.5. The embryos exhibit growth retardation and both histological evaluation and PECAM-1 staining of E9.5 embryos revealed defects in angiogenic sprouting and vascular branching morphogenesis. Large and medium-size vessel formation is not affected in these embryos. Angiogenic defects were observed in several regions of the embryo and yolk sacs. These results indicate that beta 1 integrin expression on vascular endothelial cells is crucial for embryonic angiogenesis but dispensable for vasculogenesis.

引用

页码：75 / 82

页数：8

共 25 条

[1] Extensive vasculogenesis, angiogenesis, and organogenesis precede lethality in mice lacking all αv integrins [J].