Danshen modulates Nrf2-mediated signaling pathway in cisplatin-induced renal injury

被引:23
作者
Cao, Si-si [1 ,3 ]
Yan, Miao [1 ]
Hou, Zhen-yan [1 ]
Chen, Ying [1 ,3 ]
Jiang, Yun-sheng [2 ]
Fan, Xin-rong [4 ]
Fang, Ping-fei [1 ]
Zhang, Bi-kui [1 ]
机构
[1] Cent S Univ, Dept Pharm, Xiangya Hosp 2, Changsha 410011, Hunan, Peoples R China
[2] Cent S Univ, Dept Nephrol, Xiangya Hosp 2, Changsha 410011, Hunan, Peoples R China
[3] Cent S Univ, Sch Pharmaceut Sci, Changsha 410013, Hunan, Peoples R China
[4] China Acad Chinese Med Sci, Inst Basic Theories Chinese Med, Beijing 100700, Peoples R China
基金
中国国家自然科学基金;
关键词
cisplatin; nephrotoxicity; Danshen; Nrf2; oxidative stress; ACUTE KIDNEY INJURY; INDUCED NEPHROTOXICITY; OXIDATIVE STRESS; APOPTOSIS; PROTECTS; NRF2; ACTIVATION; EXPRESSION; INJECTION; EXTRACT;
D O I
10.1007/s11596-017-1801-1
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Danshen, an efficacious agent for cardiovascular diseases, has been found to play an essential role in kidney injury. In the present study, the effect of Danshen on cisplatin-induced renal dysfunction was investigated in a mouse model. Danshen was administered to mice at a dose of 3 g/kg 4 days before and 3 days after cisplatin treatment. A single intraperitoneal injection of 20 mg/kg cisplatin was used to induce nephrotoxicity. The mice were sacrificed 72 h after cisplatin intoxication. Biochemical parameters including serum creatinine and blood urea nitrogen were analyzed. Histopathological changes of kidney tissues were detected using HE staining. Antioxidant enzymes (GSH-Px and SOD) and peroxidative product (MDA) were detected. Protein expressions of Nrf2 and its target genes including HO-1 and NQO1 were measured by Western blotting. The results showed that pretreatment with Danshen significantly reduced serum creatinine and blood urea nitrogen in the cisplatin-treated mice. Histopathological examination showed that Danshen mitigated the renal damage induced by cisplatin. Moreover, Danshen restored the activities of antioxidant enzymes (GSH-Px and SOD) and normalized the MDA contents in renal tissues. Western blotting revealed that Danshen enhanced the expressions of Nrf2 and its target genes in cisplatin-exposed mice. It was suggested that Danshen protects against the cisplatin-induced renal impairment in the mice, which is potentially associated with the upregulation of Nrf2-mediated signaling pathway.
引用
收藏
页码:761 / 765
页数:5
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