Coumarin-Based Fluorescent Probes for Super-resolution and Dynamic Tracking of Lipid Droplets

被引：131

作者：

Xu, Hongkang ^{[1
,2
]}

Zhang, Huihui ^{[1
,2
]}

Liu, Gang ^{[1
,2
]}

Kong, Lin ^{[1
,2
]}

Zhu, Xiaojiao ^{[1
,2
]}

Tian, Xiaohe ^{[3
]}

Zhang, Zhongping ^{[1
,2
]}

Zhang, Ruilong ^{[1
,2
]}

Wu, Zhichao ^{[1
,2
]}

Tian, Yupeng ^{[1
,2
]}

Zhou, Hongping ^{[1
,2
]}

机构：

[1] Anhui Univ, Coll Chem & Chem Engn, Key Lab Funct Inorgan Mat Chem Anhui Prov, Hefei 230601, Anhui, Peoples R China

[2] Anhui Univ, Anhui Prov Key Lab Chem Inorgan Organ Hybrid Func, Hefei 230601, Anhui, Peoples R China

[3] Anhui Univ, Sch Life Sci, Hefei 230601, Anhui, Peoples R China

来源：

ANALYTICAL CHEMISTRY | 2019年 / 91卷 / 01期

基金：

中国国家自然科学基金;

关键词：

AGGREGATION-INDUCED EMISSION; QUESTIONS; DISEASE; CELLS;

D O I：

10.1021/acs.analchem.8b04079

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Visualizing and dynamic tracking lipid droplets (LDs) are of great importance to biological research. Herein, two-photon absorption fluorescent small bioprobes based on lipophilic coumarin were developed, which exhibited high selectivity toward LDs in HeLa cells. Because of good biocompatibility and excellent photostability, the probes were applied to realize specific super-resolution visualization of the intracellular LDs in HeLa cells, offering us the quantitative results of the amount and diameters of LDs as well. Furthermore, the bioprobes were capable of monitoring the movements of the LDs in real time. We believe that bioprobes would provide new avenues to designing bioimaging and biological diagnosis.

引用

页码：977 / 982

页数：6

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