Upregulation of miR-151-5p promotes the apoptosis of intestinal epithelial cells by targeting brain-derived neurotrophic factor in ulcerative colitis mice

被引:2
作者
Zhou, Feng [1 ]
Chen, Lipeng [1 ]
Xu, Shan [1 ]
Si, Caijuan [1 ]
Li, Nan [1 ]
Dong, Hui [1 ]
Zheng, Peifen [1 ]
Wang, Weifeng [1 ]
机构
[1] Zhejiang Hosp, Dept Gastroenterol, Hangzhou, Zhejiang, Peoples R China
关键词
Ulcerative colitis; miR-151-5p; brain-derived neurotrophic factor; apoptosis; intestinal barrier integrity; MOUSE MODEL; BARRIER; EXPRESSION; MIGRATION; MICRORNA; PATHWAY; DISEASE;
D O I
10.1080/15384101.2022.2105905
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Ulcerative colitis (UC) is the most prevalent form of chronic inflammatory bowel disease, the etiology of which is poorly understood. This study investigated the role of miR-151-5p on UC and explored the role of brain-derived neurotrophic factor (BDNF) in a UC mouse model and cell model. A UC mouse model was engineered by dextran sulfate sodium (DSS) induction. Primary mouse intestinal epithelial cells (IECs) were isolated. Colitis mice were intraperitoneally injected with miR-151-5p antagomir and antagomir negative control, and weight loss, disease activity index, and colon length of mice were measured. Colon tissues of mice were histologically analyzed. A UC cell model was constructed by treating MODE-K cells with DSS. miR-151-5p expression in the cell model was modulated by transfection. The exogenous BDNF effect on the UC cell model and intestinal cell apoptosis, viability and proliferation was detected by flow cytometry, CCK-8 and EdU experiment. The expression of miR-151-5p and apoptosis-related proteins was assessed through q-PCR and western blotting. miR-151-5p was upregulated in the colon tissues and primary IECs of colitis mice. miR-151-5p directly inhibited the expression of BNDF. miR-151-5p upregulation promoted apoptosis in UC MODE-K cells. miR-151-5p upregulation repressed the viability of UC MODE-K cells. Exogenous BNDF treatment reversed the effect of miR-151-5p on UC MODE-K cells. miR-151-5p knockdown improved UC symptoms in mice, including alleviating weight loss, reducing disease activity index and improving colon length and damaged colon tissues. miR-151-5p contributed to intestinal epithelial cells apoptosis in colitis mice via inhibiting BNDF expression.
引用
收藏
页码:2615 / 2626
页数:12
相关论文
共 33 条
  • [31] Actin related protein 3 (ARP3) promotes apoptosis of intestinal epithelial cells in ulcerative colitis
    Zhang, Shi-Qing
    Ni, Wen-Kai
    Xiao, Ming-Bing
    Jiang, Feng
    Lu, Cui-hua
    Wang, Rui-Hua
    Ni, Run-Zhou
    [J]. PATHOLOGY RESEARCH AND PRACTICE, 2019, 215 (02) : 235 - 242
  • [32] Salmonella Infection Upregulates the Leaky Protein Claudin-2 in Intestinal Epithelial Cells
    Zhang, Yong-guo
    Wu, Shaoping
    Xia, Yinglin
    Sun, Jun
    [J]. PLOS ONE, 2013, 8 (03):
  • [33] Brain-Derived Neurotrophic Factor Modulates Intestinal Barrier by Inhibiting Intestinal Epithelial Cells Apoptosis in Mice
    Zhao, Dong-Yan
    Zhang, Wen-Xue
    Qi, Qing-Qing
    Long, Xin
    Li, Xia
    Yu, Yan-Bo
    Zuo, Xiu-Li
    [J]. PHYSIOLOGICAL RESEARCH, 2018, 67 (03) : 475 - 485