Accelerated methanogenesis from aliphatic and aromatic hydrocarbons under iron- and sulfate-reducing conditions

被引：55

作者：

Siegert, Michael ^{[1
]}

Cichocka, Danuta ^{[2
]}

Herrmann, Steffi ^{[2
]}

Gruendger, Friederike ^{[1
]}

Feisthauer, Stefan ^{[3
]}

Richnow, Hans-Hermann ^{[3
]}

Springael, Dirk ^{[2
]}

Krueger, Martin ^{[1
]}

机构：

[1] Bundesanstalt Geowissensch & Rohstoffe, D-30655 Hannover, Germany

[2] Katholieke Univ Leuven, B-3001 Heverlee, Belgium

[3] UFZ Helmholtz Ctr Environm Res, Leipzig, Germany

来源：

FEMS MICROBIOLOGY LETTERS | 2011年 / 315卷 / 01期

关键词：

methanogenic hydrocarbon degradation; microbially enhanced oil recovery; metal reduction; bioremediation; Geobacter; anaerobic oxidation of methane; ANAEROBIC BENZENE DEGRADATION; DEGRADING ENRICHMENT CULTURE; REAL-TIME PCR; MICROBIAL COMMUNITIES; CONTAMINATED AQUIFER; PETROLEUM RESERVOIR; ALKANE METABOLISM; HARBOR SEDIMENTS; MARINE-SEDIMENTS; INITIAL REACTION;

D O I：

10.1111/j.1574-6968.2010.02165.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The impact of four electron acceptors on hydrocarbon-induced methanogenesis was studied. Methanogenesis from residual hydrocarbons may enhance the exploitation of oil reservoirs and may improve bioremediation. The conditions to drive the rate-limiting first hydrocarbon-oxidizing steps for the conversion of hydrocarbons into methanogenic substrates are crucial. Thus, the electron acceptors ferrihydrite, manganese dioxide, nitrate or sulfate were added to sediment microcosms acquired from two brackish water locations. Hexadecane, ethylbenzene or 1-13C-naphthalene were used as model hydrocarbons. Methane was released most rapidly from incubations amended with ferrihydrite and hexadecane. Ferrihydrite enhanced only hexadecane-dependent methanogensis. The rates of methanogenesis were negatively affected by sulfate and nitrate at concentrations of more than 5 and 1 mM, respectively. Metal-reducing Geobacteraceae and potential sulfate reducers as well as Methanosarcina were present in situ and in vitro. Ferrihydrite addition triggered the growth of Methanosarcina-related methanogens. Additionally, methane was removed concomitantly by anaerobic methanotrophy. ANME-1 and -2 methyl coenzyme M reductase genes were detected, indicating anaerobic methanotrophy as an accompanying process [Correction added 16 December after online publication: 'methyl coenzyme A' changed to 'methyl coenzyme M' in this sentence]. The experiments presented here demonstrate the feasibility of enhancing methanogenic alkane degradation by ferrihydrite or sulfate addition in different geological settings.

引用

页码：6 / 16

页数：11