Visual and Rapid Detection of Two Genetically Modified Soybean Events Using Loop-mediated Isothermal Amplification Method

被引：107
作者：
Guan, Xiaoyan [1 ]
Guo, Jinchao [1 ]
Shen, Ping [2 ]
Yang, Litao [1 ]
Zhang, Dabing [1 ,3 ]
机构：
[1] Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, GMO Detect Lab, SJTU Bor Luh Food Safety Ctr, Shanghai 200240, Peoples R China
[2] Minist Agr, Dev Ctr Sci & Technol, Beijing 100026, Peoples R China
[3] Shanghai Jiao Tong Univ, Biox Res Ctr, Key Lab Genet & Dev & Neuropsychiat Dis, Minist Educ, Shanghai 200240, Peoples R China
来源：
FOOD ANALYTICAL METHODS | 2010年 / 3卷 / 04期
基金：
中国国家自然科学基金;
关键词：
Loop-mediated Isothermal Amplification; Visual Detection; Genetically Modified Soybean; MODIFIED ORGANISMS; QUANTIFICATION; VALIDATION; DIAGNOSIS; QUALITY; CANOLA; ASSAYS; GMOS; GENE;
D O I：
10.1007/s12161-010-9132-x
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
To develop effective alternatives for detecting genetically modified organisms (GMOs), we reported one optimized visual loop-mediated isothermal amplification (LAMP) method for the detection of exogenous DNA targets from two GM soybean events in this study. This isothermal amplification can be performed within 40 min without polymerase chain reaction (PCR) equipment and the derived LAMP products can be directly observed by naked eye employing SybrGreen I dye instead of conventional gel electrophoresis analysis. The limits of detection of these established visual LAMP assays were about 4 copies of haploid soybean genomic DNA, and which were much higher than those of reported conventional PCR assays. Furthermore, the high specificity of LAMP assays was determined. All the results demonstrated that the developed visual LAMP assays are convenient, cost-efficient, and rapid for on spot detection of GMOs.
引用
收藏
页码：313 / 320
页数：8
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