Fluidic microstructuring of alginate hydrogels for the single cell niche

被引：11

作者：

Braschler, Thomas ^{[1
]}

Valero, Ana ^{[1
]}

Colella, Ludovica ^{[1
]}

Pataky, Kristopher ^{[1
]}

Brugger, Juergen ^{[1
]}

Renaud, Philippe ^{[1
]}

机构：

[1] Ecole Polytech Fed Lausanne, Lab Microsyst, CH-1015 Lausanne, Switzerland

来源：

LAB ON A CHIP | 2010年 / 10卷 / 20期

关键词：

FLUORESCEIN DIACETATE; PHOTOLITHOGRAPHY; DIFFERENTIATION; VIABILITY; SURFACES;

D O I：

10.1039/c004988c

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Controlling alginate gel formation by diffusion of Ca2+ ions through a filter barrier, a layer-by-layer deposition technique with resolution on the size scale of a single cell is presented. It offers the possibility of exposing cells under biocompatible conditions to microheterogeneous three-dimensional environments, mimicking the layered structure of extracellular matrix in tissues.

引用

页码：2771 / 2777

页数：7

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