Long non-coding RNA NRSN2-AS1, transcribed by SOX2, promotes progression of esophageal squamous cell carcinoma by regulating the ubiquitin-degradation of PGK1

被引：11
作者：
Xu, Tongxin [1 ]
Yan, Zhaoyang [2 ]
Lu, Juntao [3 ]
Chen, Liying [3 ]
Li, Xiaoxu [3 ]
Li, Yan [3 ]
Dong, Zhiming [3 ]
Guo, Wei [3 ]
机构：
[1] Hebei Med Univ, Dept CT & MRI, Hosp 4, Shijiazhuang, Hebei, Peoples R China
[2] Hebei Med Univ, Dept Thorac Surg, Hosp 4, Shijiazhuang, Hebei, Peoples R China
[3] Hebei Med Univ, Hebei Canc Inst, Lab Pathol, Hosp 4, Jiankang Rd 12, Shijiazhuang 050011, Hebei, Peoples R China
来源：
CLINICAL & EXPERIMENTAL METASTASIS | 2022年 / 39卷 / 05期
基金：
中国国家自然科学基金;
关键词：
Esophageal squamous cell carcinoma; lncRNA NRSN2-AS1; SOX2; PGK1; Ubiquitination; CANCER PROGRESSION; PROLIFERATION; LNCRNA; EXPRESSION; ESCC; SLUG;
D O I：
10.1007/s10585-022-10174-7
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Aberrant expression of long non-coding RNAs (lncRNAs) plays pivotal roles in tumorigenesis of human malignant cancers, including esophageal squamous cell carcinoma (ESCC). However, the specific role of lncRNA NRSN2-AS1 in ESCC has not been investigated. Our analysis of clinical data revealed that NRSN2-AS1 was upregulated in ESCC tissues and negatively correlated with patient survival. Luciferase reporter assays and chromatin immunoprecipitation assays demonstrated that NRSN2-AS1 is transcribed by SOX2. In vitro functional experiments showed that NRSN2-AS1 can promote ESCC cell proliferation, migration, and invasion. Furthermore, NRSN2-AS1-binding proteins were detected using RNA pull-down assays and mass spectrometry. Mechanistically, NRSN2-AS1 can bind to phosphoglycerate kinase 1 (PGK1) and upregulate its protein levels by inhibiting its ubiquitination. Knockdown of PGK1 in part abolished the NRSN2-AS1 overexpression-induced effects on ESCC cell proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT). Thus, NRSN2-AS1 may be a diagnostic biomarker or treatment target for ESCC.
引用
收藏
页码：757 / 769
页数：13
相关论文
共 38 条
[31]   LncRNA LINRIS stabilizes IGF2BP2 and promotes the aerobic glycolysis in colorectal cancer [J].
Wang, Yun ;
Lu, Jia-Huan ;
Wu, Qi-Nian ;
Jin, Ying ;
Wang, De-Shen ;
Chen, Yan-Xing ;
Liu, Jia ;
Luo, Xiao-Jing ;
Meng, Qi ;
Pu, Heng-Ying ;
Wang, Ying-Nan ;
Hu, Pei-Shan ;
Liu, Ze-Xian ;
Zeng, Zhao-Lei ;
Zhao, Qi ;
Deng, Rong ;
Zhu, Xiao-Feng ;
Ju, Huai-Qiang ;
Xu, Rui-Hua .
MOLECULAR CANCER, 2019, 18 (01)
[32]   Extracellular ATP promotes breast cancer invasion and epithelial-mesenchymal transition via hypoxia-inducible factor 2α signaling [J].
Yang, Hui ;
Geng, Yue-Hang ;
Wang, Peng ;
Zhou, Yan-Ting ;
Yang, Han ;
Huo, Yan-Fei ;
Zhang, Hong-Quan ;
Li, Yan ;
He, Hui-Ying ;
Tian, Xin-Xia ;
Fang, Wei-Gang .
CANCER SCIENCE, 2019, 110 (08) :2456-2470
[33]   Understanding Esophageal Cancer: The Challenges and Opportunities for the Next Decade [J].
Yang, Jianjun ;
Liu, Xiguang ;
Cao, Sai ;
Dong, Xiaoying ;
Rao, Shuan ;
Cai, Kaican .
FRONTIERS IN ONCOLOGY, 2020, 10
[34]   Advances in targeted therapy for esophageal cancer [J].
Yang, Yan-Ming ;
Hong, Pan ;
Xu, Wen Wen ;
He, Qing-Yu ;
Li, Bin .
SIGNAL TRANSDUCTION AND TARGETED THERAPY, 2020, 5 (01)
[35]   Macrophage-Associated PGK1 Phosphorylation Promotes Aerobic Glycolysis and Tumorigenesis [J].
Zhang, Yajuan ;
Yu, Guanzhen ;
Chu, Huiying ;
Wang, Xiongjun ;
Xiong, Lingling ;
Cai, Guoqing ;
Liu, Ruilong ;
Gao, Hong ;
Tao, Bangbao ;
Li, Wenfeng ;
Li, Guohui ;
Liang, Ji ;
Yang, Weiwei .
MOLECULAR CELL, 2018, 71 (02) :201-+
[36]   LBX2-AS1 is activated by ZEB1 and promotes the development of esophageal squamous cell carcinoma by interacting with HNRNPC to enhance the stability of ZEB1 and ZEB2 mRNAs [J].
Zhang, Yanshan ;
Chen, Weizuo ;
Pan, Tingting ;
Wang, Huijuan ;
Zhang, Yinguo ;
Li, Chao .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2019, 511 (03) :566-572
[37]   SOX2 promotes chemoresistance, cancer stem cells properties, and epithelial-mesenchymal transition by β-catenin and Beclin1/autophagy signaling in colorectal cancer [J].
Zhu, Yin ;
Huang, Shimiao ;
Chen, Shengyuan ;
Chen, Jiaxuan ;
Wang, Zhiqing ;
Wang, Yadong ;
Zheng, Haoxuan .
CELL DEATH & DISEASE, 2021, 12 (05)
[38]   Phosphoglycerate kinase 1 a promoting enzyme for peritoneal dissemination in gastric cancer [J].
Zieker, Derek ;
Koenigsrainer, Ingmar ;
Tritschler, Isabel ;
Loeffler, Markus ;
Beckert, Stefan ;
Traub, Frank ;
Nieselt, Kay ;
Buehler, Sarah ;
Weller, Michael ;
Gaedcke, Jochen ;
Taichman, Russell S. ;
Northoff, Hinnak ;
Bruecher, Bjoern L. D. M. ;
Koenigsrainer, Alfred .
INTERNATIONAL JOURNAL OF CANCER, 2010, 126 (06) :1513-1520
1234