Identification and quantification of Chlamydia pneumoniae in human atherosclerotic plaques by LightCycler real-timer-PCR

被引：21
|
作者：
Ciervo, A [1 ]
Petrucca, A [1 ]
Cassone, A [1 ]
机构：
[1] Ist Super Sanita, Dept Bacteriol & Med Mycol, I-00161 Rome, Italy
来源：
MOLECULAR AND CELLULAR PROBES | 2003年 / 17卷 / 2-3期
关键词：
Chlamydia pneumoniae; atherosclerotic plaques; quantification; real-time PCR;
D O I：
10.1016/S0890-8508(03)00028-8
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A real-time PCR assay for Chlamydia pneumoniae in human atherosclerotic plaques by the use of novel probes and FRET LightCycler technology, is described. The assay proved particularly suitable for the specific and quantitative detection of a low DNA copy number in conventional PCR-negative samples. Among fifteen nested-PCR negative atherosclerotic plaques examined, our method detected three positive plaques containing 50(+/-3), 37(+/-2) and 24(+/-2) DNA copy number +/-SD in three independent experiments. Real-time PCR holds promise for C. pneumoniae quantitation in human atherosclerotic plaques. (C) 2003 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码：107 / 111
页数：5
相关论文
共 50 条
  • [41] A review of computational methods applied for identification and quantification of atherosclerotic plaques in images
    Jodas, Danilo Samuel
    Pereira, Aledir Silveira
    Tavares, Joao Manuel R. S.
    EXPERT SYSTEMS WITH APPLICATIONS, 2016, 46 : 1 - 14
  • [42] Quantification of mitochondrial DNA by real time Lightcycler PCR in Alzheimer's disease.
    Rodriguez-Santiago, B
    Casademont, J
    Nunes, V
    AMERICAN JOURNAL OF HUMAN GENETICS, 2000, 67 (04) : 360 - 360
  • [43] Development of a multiplex TaqMan real-time PCR assay for the detection of Chlamydia psittaci and Chlamydia pneumoniae in human clinical specimens
    Wolff, Bernard J.
    Morrison, Shatavia S.
    Winchell, Jonas M.
    DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2018, 90 (03) : 167 - 170
  • [44] Presence of Chlamydia pneumoniae in human symptomatic and asymptomatic carotid atherosclerotic plaque
    LaBiche, R
    Koziol, D
    Quinn, TC
    Gaydos, C
    Azhar, S
    Ketron, G
    Sood, S
    DeGraba, TJ
    STROKE, 2001, 32 (04) : 855 - 860
  • [45] Presence of Chlamydia Pneumoniae in human symptomatic and asymptomatic carotid atherosclerotic plaque
    DeGraba, TJ
    Azhar, S
    Ketron, G
    Sood, S
    LaBiche, R
    NEUROLOGY, 2000, 54 (07) : A139 - A139
  • [46] Immunohistochemical identification of PAPP-A in human atherosclerotic plaques
    Solovyeva, T.
    Postnikov, A.
    Serebryanaya, D.
    Tamm, N.
    Paltseva, E.
    Katrukha, A.
    CLINICAL CHEMISTRY, 2008, 54 (06) : A90 - A90
  • [47] Prevalence of Chlamydia pneumoniae Infection in Atherosclerotic Plaques of Corpses Referred to Iranian Legal Medicine Organization, Tehran
    Eskandarion, Mohammad Reza
    Nasr, Reza
    Eidgahi, Mohammad Reza Akbari
    Mozaffari, Elnaz
    Norouzi, Mehdi
    Tabasi, Mohsen
    Ghasemi, Mohammad Reza
    Gharehdaghi, Jaber
    JOURNAL OF CLINICAL AND DIAGNOSTIC RESEARCH, 2018, 12 (06) : HC01 - HC04
  • [48] Chlamydia pneumoniae in the atherosclerotic plaques of patients with coronary artery disease.: Does it have prognostic implications?
    Garcia, J
    Zamorano, J
    Moreno, R
    Suárez, A
    Culebras, E
    Castañón, J
    Gil, M
    Sánchez-Harguindey, L
    EUROPEAN HEART JOURNAL, 2002, 23 : 422 - 422
  • [49] Chlamydial heat-shock protein-60 antibody and correlation with Chlamydia pneumoniae in atherosclerotic plaques
    Fong, IW
    Chiu, B
    Viira, E
    Tucker, W
    Wood, H
    Peeling, RW
    JOURNAL OF INFECTIOUS DISEASES, 2002, 186 (10): : 1469 - 1473
  • [50] Demonstration of viable Chlamydia pneumoniae in atherosclerotic plaques of carotid arteries by reverse transcriptase polymerase chain reaction
    Esposito, G
    Blasi, F
    Allegra, L
    Chiesa, R
    Melissano, G
    Cosentini, R
    Tarsia, P
    Dordoni, L
    Cantoni, C
    Arosio, C
    Fagetti, L
    ANNALS OF VASCULAR SURGERY, 1999, 13 (04) : 421 - 425
12345