Identification and quantification of Chlamydia pneumoniae in human atherosclerotic plaques by LightCycler real-timer-PCR

被引：21
|
作者：
Ciervo, A [1 ]
Petrucca, A [1 ]
Cassone, A [1 ]
机构：
[1] Ist Super Sanita, Dept Bacteriol & Med Mycol, I-00161 Rome, Italy
来源：
MOLECULAR AND CELLULAR PROBES | 2003年 / 17卷 / 2-3期
关键词：
Chlamydia pneumoniae; atherosclerotic plaques; quantification; real-time PCR;
D O I：
10.1016/S0890-8508(03)00028-8
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A real-time PCR assay for Chlamydia pneumoniae in human atherosclerotic plaques by the use of novel probes and FRET LightCycler technology, is described. The assay proved particularly suitable for the specific and quantitative detection of a low DNA copy number in conventional PCR-negative samples. Among fifteen nested-PCR negative atherosclerotic plaques examined, our method detected three positive plaques containing 50(+/-3), 37(+/-2) and 24(+/-2) DNA copy number +/-SD in three independent experiments. Real-time PCR holds promise for C. pneumoniae quantitation in human atherosclerotic plaques. (C) 2003 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码：107 / 111
页数：5
相关论文
共 50 条
  • [21] Search for Chlamydia pneumoniae genes and their expression in atherosclerotic plaques of carotid arteries
    Valassina, M
    Migliorini, L
    Sansoni, A
    Sani, G
    Corsaro, D
    Cusi, MG
    Valensin, PE
    Cellesi, C
    JOURNAL OF MEDICAL MICROBIOLOGY, 2001, 50 (03) : 228 - 232
  • [22] Detection of Chlamydia pneumoniae but not Helicobacter pylori in atherosclerotic plaques of aortic aneurysms
    Blasi, F
    Denti, F
    Erba, M
    Cosentini, R
    Raccanelli, R
    Rinaldi, A
    Fagetti, L
    Esposito, G
    Ruberti, U
    Allegra, L
    JOURNAL OF CLINICAL MICROBIOLOGY, 1996, 34 (11) : 2766 - 2769
  • [23] Matrix metalloproteinase induction/activation system in human atherosclerotic plaques infected with Chlamydia pneumoniae: The potential role of Chlamydia pneumoniae infection in the progression of atherosclerosis
    Kwon, HM
    Choi, EY
    Kim, D
    Park, HY
    Hwang, KC
    Hong, BK
    Kim, HS
    CIRCULATION, 2003, 107 (19) : E172 - E172
  • [24] Real-time PCR for Chlamydia pneumoniae utilizing the Roche lightcycler and a 16S rRNA gene target
    Hardick, J
    Maldeis, N
    Theodore, M
    Wood, BJ
    Yang, S
    Lin, S
    Quinn, T
    Gaydos, C
    JOURNAL OF MOLECULAR DIAGNOSTICS, 2004, 6 (02): : 132 - 136
  • [25] Persistence of chlamydia pneumoniae in human atherosclerotic plaques material - Evidence and consequences [Persistenz von chlamydia pneumoniae in humanem arteriosklerotischen plaquematerial - Evidenzen und konsequenzen]
    Maass M.
    Herz, 1998, 23 (3) : 178 - 184
  • [26] Matrix metalloproteinase-9 expression is associated with the presence of Chlamydia pneumoniae in human coronary atherosclerotic plaques
    Arno, G
    Kaski, JC
    Smith, DA
    Akiyu, JP
    Hughes, SE
    Baboonian, C
    HEART, 2005, 91 (04) : 521 - 525
  • [27] Unstable atherosclerotic plaques contain T-cells that respond to Chlamydia pneumoniae
    de Boer, OJ
    van der Wal, AC
    Houtkamp, MA
    Ossewaarde, JM
    Teeling, P
    Becker, AE
    CARDIOVASCULAR RESEARCH, 2000, 48 (03) : 402 - 408
  • [28] Chlamydia pneumoniae DNA in non-coronary atherosclerotic plaques and circulating leukocytes
    Berger, M
    Schröder, B
    Daeschlein, G
    Schneider, W
    Busjahn, A
    Buchwalow, I
    Luft, FC
    Haller, H
    JOURNAL OF LABORATORY AND CLINICAL MEDICINE, 2000, 136 (03): : 194 - 200
  • [29] Normalized quantification of human cytomegalovirus DNA by competitive real-time PCR on the LightCycler instrument
    Stöcher, M
    Berg, J
    JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (12) : 4547 - 4553
  • [30] Detection of Chlamydia pneumoniae in atherosclerotic tissue:: a comparative study of PCR and immunocytochemistry
    Arno, G
    Kaski, JC
    Smith, DA
    Akiyu, J
    Zal, B
    Millar, BC
    Moore, JE
    Baboonian, C
    BRITISH JOURNAL OF BIOMEDICAL SCIENCE, 2005, 62 (04) : 155 - 160
12345