The novel putative bile acid transporter SLC10A5 is highly expressed in liver and kidney

被引：24

作者：

Fernandes, Carla F.

Godoy, Jose R.

Doering, Barbara

Cavalcanti, Marcia C. O.

Bergmann, Martin

Petzinger, Ernst

Geyer, Joachim

机构：

[1] Univ Giessen, Inst Pharmacol & Toxicol, D-35392 Giessen, Germany

[2] Univ Giessen, Inst Vet Anat Histol & Embryol, D-35390 Giessen, Germany

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2007年 / 361卷 / 01期

关键词：

SLC10A5; SLC10; NTCP; bile acid transport; liver; proximal tubules;

D O I：

10.1016/j.bbrc.2007.06.160

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Here we report the identification, cloning, and characterization of SLC10A5, which is a new member of Solute Carrier Family 10 SLC10A5, also known as the "sodium/bile acid cotransporter family". Expression of SLCIOA5/Slc10a5 was examined by quantitative real-time PCR and revealed its highest expression levels in liver and kidney in humans, rat and mouse. In rat liver and kidney, Slc10a5 expression was localized by in situ hybridization to hepatocytes and proximal tubules, respectively. A SLC10A5-FLAG fusion protein was expressed in HEK293 cells and showed an apparent molecular weight of 42 kDa after immunoprecipitation. When expressed in Xenopus laevis oocytes, the SLC10A5-FLAG protein was detected in the oocyte's plasma membrane but showed no transport activity for taurocholate, cholate, estrone-3-sulfate, or dehydroepiandrosterone sulfate. As bile acid carriers are the most related carriers to SLC10A5 though, we strongly suppose that SLC10A5 is an orphan carrier with yet non-identified substrates. (c) 2007 Elsevier Inc. All rights reserved.

引用

页码：26 / 32

页数：7

共 17 条

[1] CPG ISLANDS OF THE X-CHROMOSOME ARE GENE ASSOCIATED [J].

ALCALAY, M ;

TONIOLO, D .

NUCLEIC ACIDS RESEARCH, 1988, 16 (20) :9527-9543

[2] Comparative analysis of the ontogeny of a sodium-dependent bile acid transporter in rat kidney and ileum [J].

Christie, DM ;

Dawson, PA ;

Thevananther, S ;

Schneider, BL .

AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 1996, 271 (02) :G377-G385

[3] Expression and transport properties of the human ileal and renal sodium-dependent bile acid transporter [J].

Craddock, AL ;

Love, MW ;

Daniel, RW ;

Kirby, LC ;

Walters, HC ;

Wong, MH ;

Dawson, PA .

AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 1998, 274 (01) :G157-G169

[4] The solute carrier family SLC10: more than a family of bile acid transporters regarding function and phylogenetic relationships [J].

Geyer, J ;

Wilke, T ;

Petzinger, E .

NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY, 2006, 372 (06) :413-431

[5] Identification of a sodium-dependent organic anion transporter from rat adrenal gland [J].

Geyer, J ;

Godoy, JR ;

Petzinger, E .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2004, 316 (02) :300-306

[6]

GEYER J, 2007, J BIOL CHEM

[7] The sodium bile salt cotransport family SLC10 [J].

Hagenbuch, B ;

Dawson, P .

PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 2004, 447 (05) :566-570

[8] FUNCTIONAL EXPRESSION CLONING AND CHARACTERIZATION OF THE HEPATOCYTE NA+/BILE ACID COTRANSPORT SYSTEM [J].

HAGENBUCH, B ;

STIEGER, B ;

FOGUET, M ;

LUBBERT, H ;

MEIER, PJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (23) :10629-10633

[9] Localization and genomic organization of a new hepatocellular organic anion transporting polypeptide [J].

König, J ;

Cui, YH ;

Nies, AT ;

Keppler, D .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (30) :23161-23168

[10] A novel human organic anion transporting polypeptide localized to the basolateral hepatocyte membrane [J].

König, J ;

Cui, YH ;

Nies, AT ;

Keppler, D .

AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 2000, 278 (01) :G156-G164

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