Transcription Factor c-Maf Promotes Immunoregulation of Programmed Cell Death 1-Expressed CD8+ T Cells in Multiple Sclerosis

被引:13
作者
Koto, Shusuke [1 ]
Chihara, Norio [1 ]
Akatani, Ritsu [1 ]
Nakano, Hiroko [1 ]
Hara, Atsushi [1 ]
Sekiguchi, Kenji [1 ]
Matsumoto, Riki [1 ]
Toda, Tatsushi [2 ]
机构
[1] Kobe Univ, Grad Sch Med, Div Neurol, Kobe, Hyogo, Japan
[2] Univ Tokyo, Dept Neurol, Grad Sch Med, Tokyo, Japan
基金
日本学术振兴会;
关键词
PD-1; AUTOIMMUNITY; EXPRESSION; RECEPTOR;
D O I
10.1212/NXI.0000000000001166
中图分类号
R74 [神经病学与精神病学];
学科分类号
摘要
Background and Objectives Multiple sclerosis (MS) is an inflammatory demyelinating disease of the CNS. CD8(+) T cells are prominently found at inflammatory sites. Recent advances in understanding checkpoint molecules, including programmed cell death 1 (PD-1), expressed on CD8(+) T cells, highlight the immune regulatory roles of this T-cell subset; however, the role of CD8(+) T cells in MS is unclear. Thus, we aimed to reveal the characteristics of PD-1-expressed (PD-1(+)) CD8(+) T cells in MS. Methods We performed a cohort, case-control study for phenotyping analysis of PD-1(+)CD8(+) T cells in disease remission and flare states using CSF and peripheral blood samples of 45 patients with MS or clinically isolated syndrome and 12 healthy subjects. We further analyzed the transcriptome of sorted PD-1(+)CD8(+) T cells obtained from interferon (IFN)-beta-treated patients and validated their regulatory machinery using in vitro cell culture assays with lentiviral gene transfer. Results In the disease remission state, PD-1(+)CD8(+) T cells were decreased in the peripheral blood of patients with MS and resolved in patients treated with IFN-beta treatment who showed immune regulatory cytokine interleukin (IL)-10 expression. In the disease flare state, we found that PD-1(+)CD8(+) T cells were enriched in the CSF, which predicted a good response to subsequent IV steroid therapy. Transcriptome analysis of sorted PD-1(+)CD8(+) T cells revealed the transcription factor c-Maf as a potential major regulator of the gene module, including multiple coinhibitory molecules. Furthermore, c-Maf expressed in CD8(+) T cells induced PD-1 expression and production of IL-10 as well as suppressed alloactivated CD4(+) T-cell survival. Discussion This study uncovered a favorable role of PD-1(+)CD8(+) T cells against MS and demonstrated that c-Maf-driven IL-10 is an immune regulatory machinery.
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页数:9
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