Quadruplexes of human telomere dG3(TTAG3)3 sequences containing guanine abasic sites

被引：38

作者：

Skolakova, Petra ^{[2
]}

Bednarova, Klara ^{[2
]}

Vorlickova, Michaela ^{[2
]}

Sagi, Janos ^{[1
]}

机构：

[1] Rimstone Lab, RLI, Cheshire, CT 06410 USA

[2] Acad Sci Czech Republ, Inst Biophys, CZ-61265 Brno, Czech Republic

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2010年 / 399卷 / 02期

关键词：

Abasic site; Human telomere DNA; Quadruplex; CD spectra; Thermal stability; Thermodynamic parameters; DNA; DUPLEX; STABILITY; REPAIR; ARRANGEMENTS; 15-MER;

D O I：

10.1016/j.bbrc.2010.07.055

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This study was performed to evaluate how the loss of a guanine base affects the structure and stability of the three-tetrad G-quadruplex of 5'-dG(3)(TTAG(3))(3), the basic quadruplex-forming unit of the human telomere DNA. None of the 12 possible abasic sites hindered the formation of quadruplexes, but all reduced the thermodynamic stability of the parent quadruplex in both NaCl and KCl. The base loss did not change the Na+-stabilized intramolecular antiparallel architecture, based on CD spectra, but held up the conformational change induced in dG(3)(TTAG(3))(3) in physiological concentration of KCl. The reduced stability and the inhibited conformational transitions observed here in vitro for the first time may predict that unrepaired abasic sites in G-quadruplexes could lead to changes in the chromosome's terminal protection in vivo. (c) 2010 Elsevier Inc. All rights reserved.

引用

页码：203 / 208

页数：6

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