Expression and tissue distribution of extracellular calcium-sensing receptor (CaSR) mRNA in chickens

被引：3

作者：

Deng, Guangcun ^{[1
,2
]}

Bi, Jianmin ^{[1
]}

Qiao, Jian ^{[1
]}

He, Guiei ^{[1
]}

Wu, Boning ^{[1
]}

Zhang, Miaojie ^{[1
]}

Lv, Nana ^{[1
]}

机构：

[1] China Agr Univ, Coll Vet Med, Beijing 100193, Peoples R China

[2] Ningxia Univ Yinchuan, Coll Life Sci, Yinchuan 750021, Peoples R China

来源：

TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES | 2010年 / 34卷 / 03期

基金：

中国国家自然科学基金;

关键词：

Extracellular calcium-sensing receptor (CaSR); parathyroid hormone; tissue distribution; calcium homeostasis; CA2+-SENSING RECEPTOR; FUNCTIONAL EXPRESSION; CLONING; CA2+;

D O I：

10.3906/vet-0809-27

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Extracellular calcium-sensing receptor (CaSR) is a recently discovered G-protein-coupled receptor that may play a key role in regulating parathyroid hormone secretion and calcium homeostasis. In the present study, based on the reported sequence of the chicken CaSR gene (GenBank accession: XM_416491), specific primers were designed and synthesized, and CaSR gene mRNA expression and tissue distribution in chickens were analyzed using semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR) analysis. It was observed that CaSR mRNA was expressed in the kidney and parathyroid to a greater degree than in the small intestine, oviduct, and brain (P < 0.01). Sequence analysis of the PCR products obtained from the 5 tissues showed that these cDNAs shared 100% homology with each other and the corresponding region of the reported CaSR cDNA. The results may provide a basis and additional insight for understanding the physiological function of CaSR in chickens.

引用

页码：249 / 254

页数：6

共 30 条

[1] CLONING AND CHARACTERIZATION OF AN EXTRACELLULAR CA2+-SENSING RECEPTOR FROM BOVINE PARATHYROID [J].

BROWN, EM ;

GAMBA, G ;

RICCARDI, D ;

LOMBARDI, M ;

BUTTERS, R ;

KIFOR, O ;

SUN, A ;

HEDIGER, MA ;

LYTTON, J ;

HEBERT, SC .

NATURE, 1993, 366 (6455) :575-580

[2]

Brown EM, 1998, RECENT PROG HORM RES, V53, P257

[3] Calcium-receptor-regulated parathyroid and renal function [J].

Brown, EM ;

Hebert, SC .

BONE, 1997, 20 (04) :303-309

[4] EXTRACELLULAR CA2+ SENSING, REGULATION OF PARATHYROID CELL-FUNCTION, AND ROLE OF CA2+ AND OTHER IONS AS EXTRACELLULAR (1ST) MESSENGERS [J].

BROWN, EM .

PHYSIOLOGICAL REVIEWS, 1991, 71 (02) :371-411

[5] Extracellular calcium sensing and extracellular calcium signaling [J].

Brown, EM ;

MacLeod, RJ .

PHYSIOLOGICAL REVIEWS, 2001, 81 (01) :239-297

[6] Identification and localization of extracellular Ca2+-sensing receptor in rat intestine [J].

Chattopadhyay, N ;

Cheng, I ;

Rogers, K ;

Riccardi, D ;

Hall, A ;

Diaz, R ;

Hebert, SC ;

Soybel, DI ;

Brown, EM .

AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 1998, 274 (01) :G122-G130

[7] Biochemistry, physiology and pathophysiology of the extracellular calcium-sensing receptor [J].

Chattopadhyay, N .

INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 2000, 32 (08) :789-804

[8] Role of calcium-sensing receptor in mineral ion metabolism and inherited disorders of calcium-sensing [J].

Chattopadhyay, Naibedya ;

Brown, Edward M. .

MOLECULAR GENETICS AND METABOLISM, 2006, 89 (03) :189-202

[9] Mitochondrial localization of ERα and ERβ in human MCF7 cells [J].

Chen, JQ ;

Delannoy, M ;

Cooke, C ;

Yager, JD .

AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, 2004, 286 (06) :E1011-E1022

[10] Expression of an extracellular calcium-sensing receptor in rat stomach [J].

Cheng, I ;

Qureshi, I ;

Chattopadhyay, N ;

Qureshi, A ;

Butters, RR ;

Hall, AE ;

Cima, RR ;

Rogers, KV ;

Hebert, SC ;

Geibel, JP ;

Brown, EM ;

Soybel, DI .

GASTROENTEROLOGY, 1999, 116 (01) :118-126

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