Cytogenetic changes induced by sodium azide (NaN3) on Trigonella foenum-graecum L. seeds

被引：17
作者：
Siddiqui, S. [1 ]
Meghvansi, M. K. [1 ]
Hasan, Z. [2 ]
机构：
[1] Bundelkhand Univ, Inst Basic Sci, Dept Bot, Jhansi 284128, Uttar Pradesh, India
[2] Saifia Sci Post Grad Coll, Dept Bot, Bhopal 426001, Madhya Pradesh, India
来源：
SOUTH AFRICAN JOURNAL OF BOTANY | 2007年 / 73卷 / 04期
关键词：
chromosomal aberration; mitotic index; seed germination; sodium azide; Trigonella foenum-graecum;
D O I：
10.1016/j.sajb.2007.06.005
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Sodium azide (NaN3) still remains a popular plant mutagen. In the present investigation, its effects on the cytogenetic changes were studied in root tip cells of Trigonella foenum-graecum, which is an economically and medicinally important crop of India. Trigonella seeds were germinated after treating with various concentrations of sodium azide (0.1, 0.2, 0.3, 0.4 and 0.5%) at 24 +/- 1 degrees C for 72 It and the cytogenetic changes evaluated. The study revealed that sodium azide decreased the percentage seed germination, radicle length (at higher dose), mitotic index, and caused an increase in the chromosomal aberrations in a dose-dependent manner. Altogether, the root tip cells of the sodium azide-treated Trigonella seeds exhibited an increased incidence of chromosome stickiness, bridge formation, precocious separation and lagging chromosomes. (c) 2007 SAAB. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码：632 / 635
页数：4
相关论文
共 24 条
123