Bacteria encapsulated in layered double hydroxides: Towards an efficient bionanohybrid for pollutant degradation

被引:26
作者
Halma, Matilte [1 ,2 ]
Mousty, Christine [1 ,2 ]
Forano, Claude [1 ,2 ]
Sancelme, Martine [1 ,2 ]
Besse-Hoggan, Pascale [1 ,2 ]
Prevot, Vanessa [1 ,2 ]
机构
[1] Univ Blaise Pascal, Clermont Univ, Inst Chim Clermont Ferrand, F-63000 Clermont Ferrand, France
[2] ICCF, CNRS, UMR 6296, F-63171 Aubiere, France
关键词
Layered double hydroxides; Bacteria; Bionanohybrid; Biodegradation; Atrazine; SOL-GEL ENCAPSULATION; SP STRAIN ADP; ATRAZINE BIODEGRADATION; AMINO-ACIDS; IMMOBILIZATION; INTERCALATION; TRANSKETOLASE; REMEDIATION; ADSORPTION; HYBRID;
D O I
10.1016/j.colsurfb.2014.11.029
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
A soft chemical process was successfully used to immobilize Pseudomonas sp. strain ADP (ADP), a well-known atrazine (herbicide) degrading bacterium, within a Mg2Al-layered double hydroxide host matrix. This approach is based on a simple, quick and ecofriendly direct coprecipitation of metal salts in the presence of a colloidal suspension of bacteria in water. It must be stressed that by this process the mass ratio between inorganic and biological components was easily tuned ranging from 2 to 40. This ratio strongly influenced the biological activity of the bacteria towards atrazine degradation. The better results were obtained for ratios of 10 or lower, leading to an enhanced atrazine degradation rate and percentage compared to free cells. Moreover the biohybrid material maintained this biodegradative activity after four cycles of reutilization and 3 weeks storage at 4 degrees C. The ADP@MgAl-LDH bionanohybrid materials were completely characterized by X-ray diffraction (XRD), FTIR spectroscopy, thermogravimetric analysis and scanning and transmission electronic microscopy (SEM and TEM) evidencing the successful immobilization of ADP within the inorganic matrix. This synthetic approach could be readily extended to other microbial whole-cell immobilization of interest for new developments in biotechnological systems. (C) 2014 Elsevier B.V. All rights reserved.
引用
收藏
页码:344 / 350
页数:7
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