An immunological method to screen sex-specific proteins of bovine sperm

被引：25

作者：

Sang, L. ^{[1
]}

Yang, W. C. ^{[1
]}

Han, L. ^{[2
]}

Liang, A. X. ^{[1
]}

Hua, G. H. ^{[1
]}

Xiong, J. J. ^{[1
]}

Huo, L. J. ^{[1
]}

Yang, L. G. ^{[1
]}

机构：

[1] Huazhong Agr Univ, Coll Anim Sci & Technol, Educ Minist China, Key Lab Agr Anim Genet Breeding & Reprod, Wuhan 430070, Peoples R China

[2] Huazhong Agr Univ, Coll Vet Med, State Key Lab Agr Microbiol, Wuhan 430070, Peoples R China

来源：

JOURNAL OF DAIRY SCIENCE | 2011年 / 94卷 / 04期

关键词：

sperm; sex-specific protein; flow cytometry; bovine; SORTED SPERM; SPERMATOZOA; EXPRESSION; MEMBRANE; IDENTIFICATION; CHROMOSOME; ANTIBODIES; ANTIGENS; TECHNOLOGY; PROFILES;

D O I：

10.3168/jds.2010-3350

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

This study was designed to identify sex-specific antibodies (SSAb) in rabbit antisera against bovine sex-sorted sperm, and capture sex-specific proteins of bovine X- or Y- proteins by SSAb. The rabbit antisera against bovine X- or Y-sperm were first produced by a series of immunological approaches, and further purified through immuno-neutralization with excess sex-sorted Y- or X-sperm, respectively, to remove non-sex specific antibodies and enrich sex-specific antibodies. After removal of non-sex specific antibodies, the purified rabbit sera with enriched sex-specific antibodies were screened for sex-specific antibodies by immunofluorescence staining and flow cytometry. The results showed that 3.0, 2.2, and 4.2% of unsorted sperm, sex-sorted X-sperm, and sex-sorted Y-sperm were recognized by the purified rabbit antisera against Y-sperm, respectively, whereas 29.2, 19.7, and 3.9% of unsorted sperm, sex-sorted X-sperm, and sex-sorted Y-sperm were recognized by the purified rabbit antisera against X-sperm. These results suggested that the purified rabbit antisera against X-sperm contained SSAb that preferentially bound to sex-sorted X-sperm. Subsequently, the purified rabbit antisera against X- or Y-sperm were used to immunoprecipitate sex-specific proteins in bovine sperm proteins, and a 30-kDa protein was specifically captured by the rabbit antisera against X-sperm. In conclusion, our results implied that this 30-kDa protein might be a sex-specific protein in bovine X-sperm, which has the potential to be used in immunological procedures for sexing sperm.

引用

页码：2060 / 2070

页数：11

共 42 条

[1] Antibodies against the VRT101 laminin epitope correlate with human SLE disease activity and can be removed by extracorporeal immunoadsorption [J].