Detection of Neisseria gonorrhoeae in Palestinian women using polymerase chain reaction

被引：2

作者：

Farraj, Mohammad A. ^{[1
]}

Abusada, Gabi M. ^{[1
]}

Saleem, Abed Alraoof M. ^{[1
]}

Joaidi, Ayyub Y. ^{[1
]}

Radad, Raba M. ^{[1
]}

Atrash, Hiba N. ^{[1
]}

Sabri, Israr N. ^{[1
]}

Essawi, Tamer A. ^{[1
]}

机构：

[1] Birzeit Univ, Masters Program Clin Lab Sci, Fac Nursing & Allied Hlth Profess, Birzeit, Israel

来源：

ASIAN BIOMEDICINE | 2010年 / 4卷 / 04期

关键词：

Endocervix; Neisseria gonnorhoeae; Palestine; PCR; sexually transmitted disease; SEXUALLY-TRANSMITTED INFECTIONS; DAR-ES-SALAAM; TANZANIA;

D O I：

10.2478/abm-2010-0081

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Background: Neisseria gonorrohoeae is an exclusive human pathogen that primarily infects the urogenital epithelia. Infections caused by N. gonorrhoeae are considered the second major cause of sexually transmitted disease after Chlamydiae worldwide. Although the urethra and the uterine cervix serve as the initial sites for gonococcal infections in men and women, infection of the conjunctiva, pharynx, tendons, joints, as well as rectal mucosa are also reported. Objectives: The objectives of this study were to introduce molecular techniques such as polymerase chain reaction (PCR) to detect N. gonorrhoeae directly from endocervical swabs. In addition, it provides a picture of Neisseria gonorrohea infection among a sample of Palestinian women in West Bank. Methods: Two hundred and thirteen endocervical swabs were collected from sexually active married women with endocervical abnormalities attending healthcare clinics. DNA was extracted directly from the swabs and PCR was performed using specific primers targeting the off1 region of the genome. Results: The results obtained indicated that the percentage of positive cases of gonorrhoeae among the women tested was 1.40%. Conclusion: Implementing guidelines for comprehensive screening of men and women with more sensitive tests may improve detection and management of sexually transmitted infections.

引用

页码：637 / 640

页数：4

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