Study of light-induced formation of photodimers in the i-motif nucleic acid structure by rapid-scan FTIR difference spectroscopy and hybrid hard- and soft-modelling

被引：5
作者：
Benabou, Sanae [1 ]
Ruckebusch, Cyril [2 ]
Sliwa, Michel [2 ]
Avino, Anna [3 ]
Eritja, Ramon [3 ]
Gargallo, Raimundo [1 ]
de Juan, Anna [1 ]
机构：
[1] Univ Barcelona, Dept Chem Engn & Analyt Chem, Marti i Franques 1-11, E-08028 Barcelona, Spain
[2] Univ Lille Sci & Technol, LASIR CNRS, Cite Sci, F-59655 Villeneuve Dascq, France
[3] CSIC, Networking Ctr Bioengn Biomat & Nanomed CIBER BBN, Inst Adv Chem Catalonia IQAC, Jordi Girona 18-26, E-08034 Barcelona, Spain
来源：
PHYSICAL CHEMISTRY CHEMICAL PHYSICS | 2018年 / 20卷 / 29期
关键词：
MULTIVARIATE CURVE RESOLUTION; EXCITED-STATE DYNAMICS; PROMOTER REGION; RICH SEQUENCES; DNA; CYTOSINE; TRANSITIONS; STABILITY; FEATURES; DAMAGE;
D O I：
10.1039/c8cp00850g
中图分类号：
O64 [物理化学（理论化学）、化学物理学];
学科分类号：
070304 ; 081704 ;
摘要：
The i-motif is a DNA structure formed by cytosine-rich sequences, very relevant from a biochemical point of view and potentially useful in nanotechnology as pH-sensitive nanodevices or nanomotors. To provide a different view on the structural changes and dynamics of direct excitation processes involving i-motif structures, the use of rapid-scan FTIR spectroscopy is proposed. Hybrid hard- and soft-modelling based on the Multivariate Curve Resolution by Alternating Least Squares (MCR-ALS) algorithm has been used for the resolution of rapid-scan FTIR spectra and the interpretation of the photochemically induced time-dependent conformational changes of i-motif structures. The hybrid hard- and soft-modelling version of MCR-ALS (HS-MCR), which allows the introduction of kinetic models to describe process behavior, provides also rate constants associated with the transitions modeled. The results show that UV irradiation does not produce degradation of the studied sequences but induces the formation of photodimers. The presence of these affect much more the stability of i-motif structures formed by short sequences than that of those formed by longer sequences containing additional structural stabilizing elements, such as hairpins.
引用
收藏
页码：19635 / 19646
页数：12
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