Occupational exposure to metal-rich particulate matter modifies the expression of repair genes in foundry workers

被引:8
作者
Panjali, Zahra [1 ,2 ]
Hahad, Omar [3 ,4 ]
Rajabi, Fatemeh [5 ]
Maddah, Saeid [2 ]
Zendehdel, Rezvan [6 ]
机构
[1] Shahid Beheshti Univ Med Sci, Sch Publ Hlth & Safety, Student Res Comm, Tehran, Iran
[2] Tehran Med Sci, Sch Hlth & Med Engn, Dept Occupat Hlth & Safety, Tehran, Iran
[3] Johannes Gutenberg Univ Mainz, Univ Med Ctr, Dept Cardiol, Mainz, Germany
[4] German Ctr Cardiovasc Res DZHK, Partner Site Rhine Main, Mainz, Germany
[5] Shahid Beheshti Univ Med Sci, Sch Publ Hlth & Safety, Dept Occupat Hlth & Safety, Tehran, Iran
[6] Shahid Beheshti Univ Med Sci, Environm & Occupat Hazard Control Res Ctr, Tehran, Iran
关键词
Occupational exposure; metal-rich particulate matter; extremely low-frequency electromagnetic fields; DNA repair genes; oxidative stress; CANCER; RISK;
D O I
10.1177/07482337211021202
中图分类号
R1 [预防医学、卫生学];
学科分类号
1004 ; 120402 ;
摘要
Foundry workers are exposed to numerous occupational health hazards, which may result in increased risk of cancer, respiratory disease, and other diseases. Oxidative stress is known to be involved in the pathogenesis of such diseases. The present study aimed to investigate the association between multiple occupational exposures in foundry workers and expression of deoxyribonucleic acid (DNA) repair genes as a biomarker of oxidative DNA damage. The study sample comprised 17 foundry workers and 27 matched control subjects. Expression of 8-oxoguanine DNA glycosylase-1 (OGG1), inosine triphosphate pyrophosphate (ITPA), and MutT homolog 1 (MTH1) in peripheral blood was examined using the real-time polymerase chain reaction method. Air sampling to determine exposure to metal-rich particulate matter and measurement of extremely low-frequency electromagnetic fields (ELF-EMFs) were conducted according to the National Institute for Occupational Safety and Health standard methods. Personal air sampling revealed that occupational exposure to particulate matter exceeded the threshold limit values (TLVs) in 76% of the workstations, whereas ELF-EMF exposure appeared to be lower than the TLV. ITPA was significantly upregulated in foundry workers compared with control subjects, whereas no significant difference was observed for OGG1 and MTH1. Moreover, ITPA was strongly and positively correlated with the concentration of metal-rich particulate matter in foundry workers. No significant correlation was found between ELF-EMF exposure and expression of DNA repair genes. DNA repair gene expression may be a sensitive biomarker for occupational exposures, which suggests an involvement of oxidative stress in metal-induced toxicity. Further studies are needed to determine the role of DNA repair gene expression in response to occupational/environmental hazards.
引用
收藏
页码:504 / 512
页数:9
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