Chitooligosaccharides protect pancreatic β-cells from hydrogen peroxide-induced deterioration

被引：28

作者：

Karadeniz, Fatih ^{[1
]}

Artan, Murat ^{[1
]}

Kong, Chang-Suk ^{[2
]}

Kim, Se-Kwon ^{[1
,2
]}

机构：

[1] Pukyong Natl Univ, Dept Chem, Pusan 608737, South Korea

[2] Pukyong Natl Univ, Marine Bioproc Res Ctr, Pusan 608737, South Korea

来源：

CARBOHYDRATE POLYMERS | 2010年 / 82卷 / 01期

关键词：

Chitooligosaccharides; Pancreatic beta-cell; HIT-T15; Anti-oxidative; Hydrogen peroxide; OXIDATIVE STRESS; GLUCOSE TOXICITY; HYPERGLYCEMIA; EXPRESSION; CHITOSAN; ISLETS; RATS;

D O I：

10.1016/j.carbpol.2010.04.046

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Chitooligosaccharides (COS) of four different molecular weight ranges (below 1 kDa, 1-3 kDa, 3-5 kDa and 5-10 kDa) were investigated for their ability to protect beta-cells from damage induced by hydrogen peroxide. The viability of HIT-T15 pancreatic beta-cells was found by MIT assay to be significantly reduced after 4 h of treatment with 150 mu M H2O2. Chitooligosaccharides (500 mu g/ml) with molecular weights of 3-5 kDa raised cell viability to 58.46% following H2O2 addition. The radical scavenging effect of chitooligosaccharides was examined by dichlorofluorescein assay. In addition to free radical scavenging, the effect of chitooligosaccharides on oxidative-stress related enzymes and genes was tested. In agreement with the expression levels of related genes, results of cell viability and cellular radical scavenging assays indicate that chitooligosaccharides protect beta-cells from oxidative-stress-induced cellular damage, presumably due to radical scavenging activity. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：143 / 147

页数：5

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