Interaction of Antimicrobial Peptide Ponericin W1, Thanatin, and Mastatopara-S with Geotrichum citri-aurantii Genomic DNA

被引：6

作者：

Zhang, Hongyan ^{[1
]}

Liu, Sha ^{[1
]}

Li, Xindan ^{[1
]}

Wang, Wenjun ^{[1
]}

Deng, Lili ^{[1
,2
]}

Zeng, Kaifang ^{[1
,2
]}

机构：

[1] Southwest Univ, Coll Food Sci, Chongqing 400715, Peoples R China

[2] Southwest Univ, Food Storage & Logist Res Ctr, Chongqing 400715, Peoples R China

来源：

FOODS | 2021年 / 10卷 / 08期

基金：

中国国家自然科学基金;

关键词：

Geotrichum citri-aurantii; antimicrobial peptide; location; DNA binding; ESCHERICHIA-COLI; ANTIFUNGAL ACTIVITY; ANTIBACTERIAL; MECHANISM; MEMBRANE; IDENTIFICATION; FUNGICIDES; EFFICACY; MODE; ROT;

D O I：

10.3390/foods10081919

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Antimicrobial peptides of mastatopara-S (M-S), thanatin, and ponericin W1(P W1) were able to disrupt the membrane integrity and alter the morphology of the hyphae of Geotrichum citri-aurantii and then reduced the sour rot of citrus fruit. In order to understand the mechanisms of thanatin, P W1 and M-S other than membrane disruption, the interaction betwixt the peptides and G. citri-aurantii DNA were investigated in this research. The laser confocal microscopy found that P W1, thanatin, and M-S could penetrate the cell membrane. Gel retardation assay demonstrated that P W1, thanatin, and M-S could bind to the G. citri-aurantii genomic DNA in vitro. UV-visible spectra and fluorescence spectra analysis further confirmed that the peptides can bind to the DNA, and then insert into the base pairs in the DNA helix, followed by wrecking the double-helix structure. In addition, M-S, thanatin, and P W1 can suppress the synthesis of DNA and RNA of G. citri-aurantii.

引用

页数：13