Association of Toll-like receptor 3 and Toll-like receptor 9 single-nucleotide polymorphisms with hepatitis C virus persistence among Egyptians

被引:18
作者
Hamdy, Shaimaa [1 ]
Osman, Ahmed M. [2 ]
Zakaria, Zainab A. [1 ,3 ]
Galal, Iman [1 ]
Sobhy, Maha [1 ]
Hashem, Mohamed [1 ,4 ]
Allam, Walaa R. [1 ,5 ]
Abdel-Samiee, Mohamed [6 ]
Rewisha, Eman [6 ]
Waked, Imam [6 ]
Abdelwahab, Sayed F. [1 ,7 ,8 ]
机构
[1] Holding Co Biol Prod & Vaccines VACSERA, 51 Wizaret El Zeraa St, Giza 22311, Egypt
[2] Cairo Univ, Dept Zool, Fac Sci, Giza 12613, Egypt
[3] Heliopolis Univ Sustainable Dev, Biomed Res Lab, Fac Pharm, Cairo, Egypt
[4] Univ Maryland, Sch Med, Dept Epidemiol & Publ Hlth, Baltimore, MD 21201 USA
[5] Univ Sci & Technol, Ctr Genom, Zewail City Sci & Technol, Giza, Egypt
[6] Menoufia Univ, Dept Gastroenterol & Hepatol, Natl Liver Inst, Menoufia 32511, Egypt
[7] Menia Univ, Dept Microbiol & Immunol, Fac Med, Al Minya 61511, Egypt
[8] Taif Fac Pharm, Dept Microbiol, POB 888, At Taif 21974, Saudi Arabia
关键词
MEDIATED IMMUNE-RESPONSES; TLR9 GENE POLYMORPHISMS; HEALTH-CARE WORKERS; CELL RESPONSES; HCV INFECTION; MESSENGER-RNA; EXPRESSION; FIBROSIS; INNATE; RISK;
D O I
10.1007/s00705-018-3893-8
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Toll-like receptors (TLRs) give the innate immune system a considerable specificity for a large range of pathogens. TLR3 detects dsRNA of viruses while TLR9 recognizes bacterial and viral unmethylated CpG motifs. This study examined whether there is a potential association between single-nucleotide polymorphisms (SNPs) in the TLR3.rs3775290 (c.1377C/T), TLR9.rs5743836 (-1237T -> C) and TLR9.rs352140 (G2848A) genes and HCV infection among Egyptian patients and healthcare workers (HCWs). We enrolled 546 subjects (409 HCWs and 137 patients) divided into four groups: group 1 included 265 seronegative, aviremic subjects; group 2 included 25 seronegative, viremic subjects; group 3 included 87 subjects with spontaneously resolved HCV infection; and group 4 included 169 chronic HCV patients. All subjects were genotyped for TLR3.rs3775290, TLR9.rs5743836 and TLR9.rs352140 SNPs by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) analysis. TLR3.rs3775290 "CC" genotype was associated with chronic HCV infection, where there was a significantly greater frequency of this genotype among chronic patients when compared to subjects with spontaneously resolved infection (63.9% vs. 51.9%; p = 0.033; OR = 1.639 and 95% CI = 0.94-2.84). However, this SNP did not correlate with the HCV RNA load among the chronic subjects (p > 0.05). There was no significant difference in TLR9.rs5743836 and TLR9.rs352140 genotype distribution between groups (p > 0.05). Lack of association between the three SNPs was found, as the three SNPs are located on two different chromosomes. In conclusion, the TLR3.rs3775290 "CC" genotype was associated with HCV chronicity, while the TLR9 gene may not play a major role in HCV infection.
引用
收藏
页码:2433 / 2442
页数:10
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