Endoproteinase Pro-C-catalyzed peptide bond formation in frozen aqueous systems

被引：5
|
作者：
Haensler, M [1 ]
Jakubke, HD [1 ]
机构：
[1] Univ Leipzig, Inst Biochem, Fac Biosci Pharm & Psychol, D-04103 Leipzig, Germany
来源：
ENZYME AND MICROBIAL TECHNOLOGY | 1998年 / 22卷 / 07期
关键词：
endoproteinase Pro-C; Flavobacterium meningosepticum; peptide bond; frozen aqueous system;
D O I：
10.1016/S0141-0229(98)00260-9
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The capability of endoproteinase Pro-C from Flavobacterium meningosepticum to form peptide bonds in frozen aqueous systems was investigated. In coupling of Bz-Gly-Pro-OMe with various amino acid amides, Si ee amino acids, and dipeptides, freezing-induced yield enhancement strongly depended on the nucleophilic amino component ranging from 3-59%. From the different influence of freezing, it can be concluded that freeze concentration is not the only cause of yield enhancement in frozen aqueous systems. Endoproteinase Pro-C proved to be a suitable catalyst in Pro-Leu bond formation but its scope of capability ill peptide synthesis is limited. (C) 1998 Elsevier Science Inc.
引用
收藏
页码：617 / 620
页数：4
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