Identification of mNET1 as a candidate ligand for the first PDZ domain of MAGI-1

被引：42
作者：
Dobrosotskaya, IY [1 ]
机构：
[1] Univ Texas, SW Med Ctr, Dept Mol Genet, Grad Program Cell Regulat, Dallas, TX 75390 USA
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2001年 / 283卷 / 04期
关键词：
MAGI-1; NET1; MAGUK; cell junction; small G protein; Rho;
D O I：
10.1006/bbrc.2001.4880
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
This paper reports the identification of a Rho family nucleotide exchange factor termed mNET1 as a candidate-interacting partner for the first PDZ domain of MAGI-1, a membrane-associated guanylate kinase with inverted arrangement of protein-protein interacting modules, mNET1 was identified in a yeast two-hybrid screen and has a consensus tripeptide for PDZ domain binding at its extreme carboxy-terminus. In addition to this sequence, a cluster of basic residues located near the carboxy terminus is essential for the binding. The interaction of the first PDZ domain of MAGI-1 with mNET1 was documented using a variety of biochemical methods. (C) 2001 Academic Press.
引用
收藏
页码：969 / 975
页数：7
相关论文
共 32 条
  • [1] Activation of RhoA and SAPK/JNK signalling pathways by the RhoA-specific exchange factor mNET1
    Alberts, AS
    Treisman, R
    [J]. EMBO JOURNAL, 1998, 17 (14) : 4075 - 4085
  • [2] Analysis of RhoA-binding proteins reveals an interaction domain conserved in heterotrimeric G protein β subunits and the yeast response regulator protein Skn7
    Alberts, AS
    Bouquin, N
    Johnston, LH
    Treisman, R
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (15) : 8616 - 8622
  • [3] Bartel P, 1993, CELLULAR INTERACTION, P153
  • [4] Chan AML, 1996, ONCOGENE, V12, P1259
  • [5] PDZ-GEF1, a guanine nucleotide exchange factor specific for Rap1 and Rap2
    de Rooij, J
    Boenink, NM
    van Triest, M
    Cool, RH
    Wittinghofer, A
    Bos, JL
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (53) : 38125 - 38130
  • [6] MAGI-1, a membrane-associated guanylate kinase with a unique arrangement of protein-protein interaction domains
    Dobrosotskaya, I
    Guy, RK
    James, GL
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (50) : 31589 - 31597
  • [7] MAGI-1 interacts with β-catenin and is associated with cell-cell adhesion structures
    Dobrosotskaya, IY
    James, GL
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2000, 270 (03) : 903 - 909
  • [8] Crystal structures of a complexed and peptide-free membrane protein-binding domain: Molecular basis of peptide recognition by PDZ
    Doyle, DA
    Lee, A
    Lewis, J
    Kim, E
    Sheng, M
    MacKinnon, R
    [J]. CELL, 1996, 85 (07) : 1067 - 1076
  • [9] Leukemia-associated Rho guanine nucleotide exchange factor (LARG) links heterotrimeric G proteins of the G12 family to Rho
    Fukuhara, S
    Chikumi, H
    Gutkind, JS
    [J]. FEBS LETTERS, 2000, 485 (2-3) : 183 - 188
  • [10] A novel PDZ domain containing guanine nucleotide exchange factor links heterotrimeric G proteins to Rho
    Fukuhara, S
    Murga, C
    Zohar, M
    Igishi, T
    Gutkind, JS
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (09) : 5868 - 5879
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