Purification and characterization of Chromatium vinosum GroEL and GroES proteins overexpressed in Escherichia coli cells lacking the endogenous groESL operon

被引:3
作者
Dionisi, HM [1 ]
Viale, AM [1 ]
机构
[1] Univ Nacl Rosario, Fac Ciencias Bioquim & Farmaceut, Dept Microbiol, CONICET,Programa Multidisciplinario Biol Expt, RA-2000 Rosario, Argentina
关键词
GroES; GroEL; chaperonins; Chromatium vinosum; molecular chaperones;
D O I
10.1006/prep.1998.0953
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Using an Escherichia coli strain (RF101) in which the endogenous chromosomal groESL operon was removed, we overexpressed the GroEL and GroES chaperonins cloned from the photosynthetic bacterium Chromatium vinosum. The identities of these proteins were confirmed by immunological and N-terminal sequence analyses. The native molecular masses of GroEL and GroES, as determined by size exclusion chromatography, were 830 and 74 kDa, respectively. This suggests a tetradecameric structure for GroEL and a heptameric structure for GroES. C. vinosum GroEL catalyzed a K+-stimulated ATP hydrolysis with a specific activity at 25 degrees C of 50.2 +/- 3.8 nmol Pi released min(-1) mg protein(-1). GroEL ATPase was inhibited by GroES, reaching about 50% inhibition at a ratio GroES-7mer/GroEL-14mer of 1 in the presence of 10 mM KCl. The ATPase V-max increased almost fivefold in the 25 to 65 degrees C temperature range; higher temperatures led to a rapid inactivation of this activity. The chaperone activity of the C. vinosum GroEL/GroES system was characterized by its effect on the refolding of guanidinium chloride-unfolded rhodanese. In the presence of ATP and GroES, C. vinosum GroEL assisted rhodanese refolding. The heterologous combination C. vinosum GroEL/E. coli GroES or E. coli GroEL/C. vinosum GroES was as effective as the homologous complexes. In summary, this strategy allowed the purification at high yields of fully functional, homogenous C. vinosum GroEL and GroES chaperonins from E. coli. (C) 1998 Academic Press.
引用
收藏
页码:275 / 282
页数:8
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