Polyvinylpyrrolidone modified bioactive glass fibers as tissue constructs:: In vitro mesenchymal stem cell response

The sol-gel synthesis of a bioactive glass (BAG) sol with the incorporation of polyvinylpyrrolidone and the subsequent spraying of short, discontinuous fibers is reported. The incorporation of the polymer into the BAG sol allowed for increased control of the rheological properties and resulted in a more homogeneous fibrous material when sprayed through an air gun. Reaction kinetics and sol viscosity were monitored and analyzed during synthesis, and fibers were characterized using scanning electron microscopy and thermal analysis. Fibers were sintered at 900degreesC and were examined for in vitro bioactivity in a simulated body fluid solution. The presence of hydroxyapatite crystals is confirmed by examination with scanning electron microscopy, energy dispersive X-ray spectroscopy (EDX), and Xray diffraction (XRD). Both the proliferation rate and cell density of rat mesenchymal stem cells cultured on BAG fiber constructs of varying porosities were shown to be dependent upon fiber spacing. (C) 2003 Wiley Periodicals, Inc.