Crystallization and preliminary X-ray crystallographic study of the viral Zα domain bound to left-handed Z-DNA

被引：2

作者：

Ha, SC

Oh, DB

Kim, KK ^{[1
]}

Kim, YG

机构：

[1] Chung Ang Univ, Coll Med, Dept Biochem, Seoul 156756, South Korea

[2] ISU Chem Co, Biotech R&D Ctr, Seoul 120749, South Korea

[3] Sungkyunkwan Univ, Sch Med, Samsung Biomed Res Inst, Dept Mol Cell Biol, Suwon 440746, South Korea

来源：

PROTEIN AND PEPTIDE LETTERS | 2005年 / 12卷 / 04期

关键词：

Z alpha motif; poxvirus; viral protein; Z-DNA; crystallization;

D O I：

10.2174/0929866053765608

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Za domain (yabaZ alpha(E3L)) of the EM protein homologue from Yaba-like disease virus, a Yaba-virus, was co-crystallized with d(TCGCGCG)(2) in the Z-conformation. The crystals belong to the P2(1)2(1)2 space group, with unit-cell parameters a=51.20 angstrom, b=92.45 angstrom, c=48.02 angstrom, alpha=beta= gamma=-90 degrees. The diffraction data were collected up to a resolution of 2.2 angstrom. The structure of viral Z alpha motif will provide an insight into how diverse Z alpha motifs recognize Z-DNA.

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页码：391 / 393

页数：3

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